温度振荡对昆虫细胞生长和杆状病毒复制的影响。
Effect of temperature oscillation on insect cell growth and baculovirus replication.
作者信息
Shao-Hua C, Hong-Liang S, Zuo-Hu L
机构信息
State Key Laboratory of Biochemical Engineering, Institute of Chemical Metallurgy, Chinese Academy of Sciences, Beijing 100080, People's Republic of China.
出版信息
Appl Environ Microbiol. 1998 Jun;64(6):2237-9. doi: 10.1128/AEM.64.6.2237-2239.1998.
Temperature oscillation can enhance cell viability of sf9 insect cells and baculovirus production of occlusion bodies (OB) and extracellular virus (ECV) compared with constant temperature in stationary culture and suspension culture. The optimal oscillation range was 24 to 28 degreesC. At this temperature oscillation, the viability of uninfected and infected sf9 cells can be maintained much longer than at 28 degreesC. Although the rate of virus infection was a little low at 24 to 28 degreesC, the final cell infectivity was similar to that at a constant temperature of 28 degreesC. The production of OB was increased from 13.4 to 17.4/cell in stationary culture and from 13.9/cell to 18.1/cell in suspension culture. The titer of ECV was increased from 87 to 114 PFU/cell in stationary culture and from 79 to 114 PFU/cell in suspension culture.
与固定培养和悬浮培养中的恒温条件相比，温度振荡可提高sf9昆虫细胞的活力以及杆状病毒产生的包涵体（OB）和细胞外病毒（ECV）的产量。最佳振荡范围为24至28摄氏度。在此温度振荡条件下，未感染和感染的sf9细胞的活力维持时间可比在28摄氏度时长很多。尽管在24至28摄氏度时病毒感染率略低，但最终细胞感染性与28摄氏度恒温时相似。在固定培养中，OB产量从每个细胞13.4个增加到17.4个，在悬浮培养中从每个细胞13.9个增加到18.1个。在固定培养中，ECV滴度从每细胞87个空斑形成单位（PFU）增加到114个PFU，在悬浮培养中从每细胞79个PFU增加到114个PFU。
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