表观遗传对卵母细胞发育能力的影响：卵泡周围血管生成与卵泡内氧气
Epigenetic influences on oocyte developmental competence: perifollicular vascularity and intrafollicular oxygen.
作者信息
Van Blerkom J
机构信息
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder 80309-0347, USA.
出版信息
J Assist Reprod Genet. 1998 May;15(5):226-34. doi: 10.1023/a:1022523906655.
PURPOSE
Studies indicating that the developmental competence of the human oocyte is influenced by the level of intrafollicular oxygen are described.
METHODS
Perifollicular vascularity and dissolved oxygen content were determined by color Doppler ultrasonography and analysis of follicular fluid at ovum retrieval, respectively, in stimulated cycles for in vitro fertilization.
RESULTS
Differences in the degree of perifollicular vascularity correlate with differences in the dissolved oxygen content of the corresponding follicular fluid. Oocytes with cytoplasmic and chromosomal disorders and embryos with multinucleated blastomeres and limited developmental ability were derived predominantly from underoxygenated follicles. Findings from several studies indicate that embryos with the highest implantation potential originate from follicles that are well-vascularized and oxygenated.
CONCLUSIONS
Follicular vascularity and oxygen content appear to be important determinants of oocyte competence. Possible causes of differences in follicle-specific vascularity and the potential effects of severe hypoxia on the normality of molecular and cellular processes during follicle growth and preovulatory development are discussed.
目的
描述表明人类卵母细胞发育能力受卵泡内氧水平影响的研究。
方法
在体外受精的促排卵周期中，分别通过彩色多普勒超声检查和取卵时卵泡液分析来测定卵泡周围血管分布和溶解氧含量。
结果
卵泡周围血管分布程度的差异与相应卵泡液中溶解氧含量的差异相关。具有细胞质和染色体异常的卵母细胞以及具有多核卵裂球和有限发育能力的胚胎主要来自低氧卵泡。多项研究结果表明，具有最高着床潜力的胚胎源自血管化良好且含氧充足的卵泡。
结论
卵泡血管分布和氧含量似乎是卵母细胞能力的重要决定因素。讨论了卵泡特异性血管分布差异的可能原因以及严重缺氧对卵泡生长和排卵前发育过程中分子和细胞过程正常性的潜在影响。
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