通过比较基因组杂交技术检测原发性结直肠癌中的基因变化。

Genetic changes in primary colorectal cancer by comparative genomic hybridization.

作者信息

Nakao K, Shibusawa M, Tsunoda A, Yoshizawa H, Murakami M, Kusano M, Uesugi N, Sasaki K

机构信息

Second Department of Surgery, Showa University School of Medicine, Tokyo, Japan.

出版信息

Surg Today. 1998;28(5):567-9. doi: 10.1007/s005950050185.

DOI:10.1007/s005950050185

PMID:9607913

Abstract

Comparative genomic hybridization (CGH) is a powerful new technique for the molecular cytogenetic analysis of cancer. In this method, at first the cancer DNA and normal DNA are labeled with biotin and digoxigenin, respectively, and then the labeled DNAs are applied onto normal lymphocyte metaphase preparations in hybridization. After hybridization, they are stained with FITC and rhodamine, respectively, so chromosomal gains and losses in cancer are thus detected by using a green:red ratio. In this study, we analyzed the abnormal chromosomes in nine cases with human primary colon cancer. A gain in chromosomes 11p, 12q, 16p, 20p, and 20q were observed, while a loss of 18q and 22q were discovered. CGH may thus provide us with important information for analyzing the genes in colon cancer.

摘要

比较基因组杂交（CGH）是一种用于癌症分子细胞遗传学分析的强大新技术。在该方法中，首先分别用生物素和地高辛配基标记癌DNA和正常DNA，然后将标记的DNA应用于杂交中的正常淋巴细胞中期制备物上。杂交后，分别用异硫氰酸荧光素（FITC）和罗丹明染色，从而通过绿色：红色比例检测癌症中的染色体增减。在本研究中，我们分析了9例人类原发性结肠癌患者的异常染色体。观察到11p、12q、16p、20p和20q染色体增加，同时发现18q和22q染色体缺失。因此，CGH可为我们分析结肠癌中的基因提供重要信息。