破骨细胞分化因子介导了在骨微环境中由1α,25 - 二羟基维生素D3、前列腺素E2或甲状旁腺激素诱导的骨吸收所必需的信号。
Osteoclast differentiation factor mediates an essential signal for bone resorption induced by 1 alpha,25-dihydroxyvitamin D3, prostaglandin E2, or parathyroid hormone in the microenvironment of bone.
作者信息
Tsukii K, Shima N, Mochizuki S, Yamaguchi K, Kinosaki M, Yano K, Shibata O, Udagawa N, Yasuda H, Suda T, Higashio K
机构信息
Research Institute of Life Science, Snow Brand Milk Products Co., Ltd., Tochigi, Japan.
出版信息
Biochem Biophys Res Commun. 1998 May 19;246(2):337-41. doi: 10.1006/bbrc.1998.8610.
Osteoclast differentiation factor (ODF), a ligand for osteoprotegerin (OPG)/osteoclastogenesis-inhibitory factor (OCIF), induces osteoclast-like cell formation in vitro. To elucidate the role of ODF in the microenvironment of bone, we examined effects of ODF, OPG/OCIF, and anti-ODF polyclonal antibody on bone resorption using a fetal mouse long bone culture system. A genetically engineered soluble-form ODF (sODF) elicited bone resorption in a concentration-dependent manner and OPG/OCIF blocked the bone resorption. Anti-ODF polyclonal antibody, which neutralizes ODF activity, negated bone resorption induced by 1 alpha,25-dihydroxyvitamin D3, parathyroid hormone, or prostaglandin E2. OPG/OCIF also abolished bone-resorbing activity elicited by these bone-resorbing agents. Interleukin 1 alpha-stimulated bone resorption was also significantly suppressed by anti-ODF polyclonal antibody and OPG/OCIF. Thus, we conclude that ODF plays a critical role in bone resorption in the microenvironment of bone.
破骨细胞分化因子（ODF）是骨保护素（OPG）/破骨细胞生成抑制因子（OCIF）的配体，可在体外诱导破骨细胞样细胞形成。为阐明ODF在骨微环境中的作用，我们使用胎鼠长骨培养系统研究了ODF、OPG/OCIF和抗ODF多克隆抗体对骨吸收的影响。基因工程可溶性形式的ODF（sODF）以浓度依赖方式引起骨吸收，而OPG/OCIF可阻断骨吸收。中和ODF活性的抗ODF多克隆抗体可消除由1α,25 - 二羟维生素D3、甲状旁腺激素或前列腺素E2诱导的骨吸收。OPG/OCIF也消除了这些骨吸收剂引起的骨吸收活性。抗ODF多克隆抗体和OPG/OCIF也显著抑制了白细胞介素1α刺激的骨吸收。因此，我们得出结论，ODF在骨微环境中的骨吸收中起关键作用。
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