接受口服20(S)-喜树碱患者中总20(S)-喜树碱和内酯型20(S)-喜树碱的高效液相色谱定量分析
High-performance liquid chromatographic quantitation of total and lactone 20(S)camptothecin in patients receiving oral 20(S)camptothecin.
作者信息
Ahmed F, Vyas V, Saleem A, Li X G, Zamek R, Cornfield A, Haluska P, Ibrahim N, Rubin E H, Gupta E
机构信息
UMDNJ--The Cancer Institute of New Jersey, New Brunswick 08901, USA.
出版信息
J Chromatogr B Biomed Sci Appl. 1998 Apr 10;707(1-2):227-33. doi: 10.1016/s0378-4347(97)00615-4.
We have developed a sensitive high-performance liquid chromatography (HPLC) assay to quantitate total and lactone forms of 20(S)camptothecin (CPT) in human plasma. Lactone and total CPT were extracted using solid-phase extraction and liquid-liquid extraction, respectively. The extracted lactone samples could be stored without immediate HPLC analysis. The two forms of CPT were quantitated by reversed-phase HPLC with fluorescence detection. The extraction efficiencies were about 100% and 92% for the total and lactone forms, respectively. The lower limit of quantitation was 5.74 nM for the two forms. The method was reproducible with a mean interday and intraday variability of 6% for total CPT and 4% and 6%. respectively, for lactone CPT. The assay could effectively quantitate lactone and total CPT in patients receiving single dose and multiple doses of oral CPT.
我们开发了一种灵敏的高效液相色谱（HPLC）分析法，用于定量测定人血浆中20（S）-喜树碱（CPT）的总形式和内酯形式。分别采用固相萃取和液液萃取法提取内酯形式和总CPT。提取的内酯样品无需立即进行HPLC分析即可储存。通过反相HPLC和荧光检测法定量测定CPT的两种形式。总形式和内酯形式的提取效率分别约为100%和92%。两种形式的定量下限均为5.74 nM。该方法具有可重复性，总CPT的日间和日内平均变异系数分别为6%，内酯CPT分别为4%和6%。该分析法可有效定量接受单剂量和多剂量口服CPT患者体内的内酯形式和总CPT。
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