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诊断实验室中白喉棒状杆菌毒素产生的检测。

The detection of toxin production by Corynebacterium diphtheriae in diagnostic laboratories.

作者信息

Young P S, Smith D D

出版信息

Pathology. 1976 Apr;8(2):101-4. doi: 10.3109/00313027609094434.

Abstract

In vitro tests for assessing the toxigenicity of strains of C. diphtheriae using the original agar plate precipitin method of Elek are unreliable. At high concentrations of antitoxin (500-1000 U/ml) multiple non-specific precipitin lines occur making interpretation difficult. Increased specificity was obtained by diluting the antitoxin but this in turn caused a delay in the appearance of toxin-antitoxin lines. Also, the failure of lines to appear on media enriched with unsuitable serum may lead to false-negative results. Final assessment of the toxigenicity of strains of C. diphtheriae can probably only be made reliably by guinea-pig tests.

摘要

采用埃lek氏原始琼脂平板沉淀法评估白喉杆菌菌株产毒性的体外试验不可靠。在高浓度抗毒素(500 - 1000 U/ml)时,会出现多条非特异性沉淀线,难以进行解读。通过稀释抗毒素可提高特异性,但这反过来又会导致毒素 - 抗毒素线出现延迟。此外,在富含不合适血清的培养基上未出现沉淀线可能会导致假阴性结果。白喉杆菌菌株产毒性的最终评估可能只能通过豚鼠试验可靠地进行。

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