Moritaka T, Kiura K, Ueoka H, Tabata M, Segawa Y, Shibayama T, Takigawa N, Ohnoshi T, Harada M
Second Department of Medicine, Okayama University Medical School, Japan.
Anticancer Res. 1998 Mar-Apr;18(2A):927-33.
A cisplatin-resistant cell line, SBC-3/CDDP, was established from a human small-cell lung cancer cell line, SBC-3. The SBC-3/CDDP cells were 13.1-fold more resistant to cisplatin than the parent SBC-3 cells. We investigated the cellular changes of this cell line with regard to the development of resistance to cisplatin. The SBC-3/CDDP cells showed various characteristics as follows: a) increased intracellular glutathione and glutathione S-transferase content b) decreased intracellular accumulation of cisplatin, c) increased topoisomerase I activity and the same topoisomerase II activity as the parent SBC-3 cells, and 4) strong cross-resistance to the platinum analogues and mitomycin C, moderate cross-resistance to 7-ethyl-10-hydroxy-camptothecin (SN-38), 4-hydroperoxy cyclophosphamide, etoposide, Adriamycin and methotrexate, and collateral sensitivity to vinca alkaloids and 5-fluorouracil. From these observations, the SBC-3/CDDP cells could be useful as a well characterized cisplatin-resistant cell line, and the resistance pattem in this cell line will give us much information for eradication of cisplatin-resistant tumor cells.
一种顺铂耐药细胞系SBC - 3/CDDP，是从人小细胞肺癌细胞系SBC - 3建立而来。SBC - 3/CDDP细胞对顺铂的耐药性比亲代SBC - 3细胞高13.1倍。我们研究了该细胞系在对顺铂耐药性发展方面的细胞变化。SBC - 3/CDDP细胞表现出以下各种特征：a）细胞内谷胱甘肽和谷胱甘肽S - 转移酶含量增加；b）细胞内顺铂积累减少；c）拓扑异构酶I活性增加，拓扑异构酶II活性与亲代SBC - 3细胞相同；以及d）对铂类类似物和丝裂霉素C有强烈的交叉耐药性，对7 - 乙基 - 10 - 羟基喜树碱（SN - 38）、4 - 氢过氧环磷酰胺、依托泊苷、阿霉素和甲氨蝶呤有中度交叉耐药性，对长春花生物碱和5 - 氟尿嘧啶有旁侧敏感性。基于这些观察结果，SBC - 3/CDDP细胞可作为一种特征明确的顺铂耐药细胞系，该细胞系中的耐药模式将为根除顺铂耐药肿瘤细胞提供许多信息。
