棕色固氮菌谷氨酰胺合成酶基因-铵离子转运蛋白基因操纵子的特性分析
Characterization of the glnK-amtB operon of Azotobacter vinelandii.
作者信息
Meletzus D, Rudnick P, Doetsch N, Green A, Kennedy C
机构信息
Department of Plant Pathology, College of Agriculture, University of Arizona, Tucson 85721, USA.
出版信息
J Bacteriol. 1998 Jun;180(12):3260-4. doi: 10.1128/JB.180.12.3260-3264.1998.
Abstract
To determine whether in Azotobacter vinelandii the PII protein influences the regulation of nif gene expression in response to fluxes in the ammonium supply, the gene encoding PII was isolated and characterized. Its deduced translation product was highly similar to PII proteins from other organisms, with the greatest degree of relatedness being exhibited to the Escherichia coli glnK gene product. A gene designated amtB was found downstream of and was contranscribed with glnK as in E. coli. The AmtB protein is similar to functionally characterized ammonium transport proteins from a few other eukaryotes and one other prokaryote. glnK and amtB comprise an operon. Attempts to isolate a stable glnK mutant strain were unsuccessful, suggesting that glnK, like glnA, is an essential gene in A. vinelandii. amtB mutants were isolated, and although growth on limiting amounts of ammonium was similar in the mutant and wild-type strains, the mutants were unable to transport [14C]methylammonium.
摘要
为了确定在棕色固氮菌中PII蛋白是否响应铵供应通量影响固氮基因表达的调控，编码PII的基因被分离并进行了表征。其推导的翻译产物与来自其他生物的PII蛋白高度相似，与大肠杆菌glnK基因产物的相关性最高。发现一个名为amtB的基因位于glnK下游，并且与glnK共转录，如同在大肠杆菌中一样。AmtB蛋白类似于来自其他一些真核生物和另一种原核生物的功能已得到表征的铵转运蛋白。glnK和amtB组成一个操纵子。分离稳定的glnK突变株的尝试未成功，这表明glnK与glnA一样，是棕色固氮菌中的一个必需基因。分离出了amtB突变体，尽管突变体和野生型菌株在有限量铵上的生长相似，但突变体无法转运[14C]甲基铵。
相似文献
1
Characterization of the glnK-amtB operon of Azotobacter vinelandii.棕色固氮菌谷氨酰胺合成酶基因-铵离子转运蛋白基因操纵子的特性分析
J Bacteriol. 1998 Jun;180(12):3260-4. doi: 10.1128/JB.180.12.3260-3264.1998.
2
Characterisation of the glnK-amtB operon and the involvement of AmtB in methylammonium uptake in Azorhizobium caulinodans.茎瘤固氮根瘤菌中谷氨酰胺合成酶基因-铵离子转运蛋白基因操纵子的特性及铵离子转运蛋白B在甲基铵摄取中的作用
Mol Gen Genet. 1998 Jun;258(6):671-7. doi: 10.1007/s004380050781.
3
Role of Escherichia coli nitrogen regulatory genes in the nitrogen response of the Azotobacter vinelandii NifL-NifA complex.大肠杆菌氮调节基因在棕色固氮菌NifL-NifA复合物氮响应中的作用。
J Bacteriol. 2001 May;183(10):3076-82. doi: 10.1128/JB.183.10.3076-3082.2001.
4
Sensing nitrogen limitation in Corynebacterium glutamicum: the role of glnK and glnD.谷氨酸棒杆菌中氮限制的感知：glnK和glnD的作用。
Mol Microbiol. 2001 Dec;42(5):1281-95. doi: 10.1046/j.1365-2958.2001.02694.x.
5
Distinct roles of P(II)-like signal transmitter proteins and amtB in regulation of nif gene expression, nitrogenase activity, and posttranslational modification of NifH in Azoarcus sp. strain BH72.类P(II)信号转导蛋白和amtB在调控偶氮弧菌属BH72菌株中nif基因表达、固氮酶活性及NifH翻译后修饰中的不同作用
J Bacteriol. 2002 Apr;184(8):2251-9. doi: 10.1128/JB.184.8.2251-2259.2002.
6
An alternative PII protein in the regulation of glutamine synthetase in Escherichia coli.大肠杆菌中谷氨酰胺合成酶调控中的一种替代PII蛋白。
Mol Microbiol. 1996 Jul;21(1):133-46. doi: 10.1046/j.1365-2958.1996.6281349.x.
7
Lethality of glnD null mutations in Azotobacter vinelandii is suppressible by prevention of glutamine synthetase adenylylation.棕色固氮菌中glnD基因无效突变的致死性可通过防止谷氨酰胺合成酶腺苷酸化来抑制。
Microbiology (Reading). 2001 May;147(Pt 5):1267-1276. doi: 10.1099/00221287-147-5-1267.
8
Role of the GlnK signal transduction protein in the regulation of nitrogen assimilation in Escherichia coli.谷氨酰胺激酶信号转导蛋白在大肠杆菌氮同化调节中的作用。
Mol Microbiol. 1998 Jul;29(2):431-47. doi: 10.1046/j.1365-2958.1998.00932.x.
9
Antagonism of PII signalling by the AmtB protein of Escherichia coli.大肠杆菌AmtB蛋白对PII信号的拮抗作用。
Mol Microbiol. 2003 May;48(4):1017-28. doi: 10.1046/j.1365-2958.2003.03479.x.
10
Role of GlnK in NifL-mediated regulation of NifA activity in Azotobacter vinelandii.谷氨酰胺激酶（GlnK）在棕色固氮菌中NifL介导的NifA活性调控中的作用
J Bacteriol. 2002 Feb;184(3):812-20. doi: 10.1128/JB.184.3.812-820.2002.
引用本文的文献
1
Enhanced extracellular ammonium release in the plant endophyte through genome editing.通过基因组编辑增强植物内生菌的细胞外铵释放。
Microbiol Spectr. 2024 Jan 11;12(1):e0247823. doi: 10.1128/spectrum.02478-23. Epub 2023 Dec 1.
2
Molecular Mechanism and Agricultural Application of the NifA-NifL System for Nitrogen Fixation.NifA-NifL 系统固氮的分子机制及其在农业中的应用。
Int J Mol Sci. 2023 Jan 4;24(2):907. doi: 10.3390/ijms24020907.
3
Gluconacetobacter diazotrophicus Gene Fitness during Diazotrophic Growth.在固氮生长过程中，谷氨酸醋酸杆菌的基因适应性。
Appl Environ Microbiol. 2022 Dec 13;88(23):e0124122. doi: 10.1128/aem.01241-22. Epub 2022 Nov 14.
4
Control of nitrogen fixation and ammonia excretion in Azorhizobium caulinodans.根瘤菌属控制氮固定和氨排泄。
PLoS Genet. 2022 Jun 21;18(6):e1010276. doi: 10.1371/journal.pgen.1010276. eCollection 2022 Jun.
5
Key factors affecting ammonium production by an Azotobacter vinelandii strain deregulated for biological nitrogen fixation.影响弗氏固氮菌生物固氮去调控产铵的关键因素。
Microb Cell Fact. 2020 May 19;19(1):107. doi: 10.1186/s12934-020-01362-9.
6
Gene Deletions Resulting in Increased Nitrogen Release by Azotobacter vinelandii: Application of a Novel Nitrogen Biosensor.基因缺失导致维涅兰德固氮菌氮释放增加：新型氮生物传感器的应用
Appl Environ Microbiol. 2015 Jul;81(13):4316-28. doi: 10.1128/AEM.00554-15. Epub 2015 Apr 17.
7
NasT-mediated antitermination plays an essential role in the regulation of the assimilatory nitrate reductase operon in Azotobacter vinelandii.NasT 介导的反终止在根瘤菌中同化硝酸盐还原酶操纵子的调控中起着至关重要的作用。
Appl Environ Microbiol. 2012 Sep;78(18):6558-67. doi: 10.1128/AEM.01720-12. Epub 2012 Jul 6.
8
NtrC-dependent regulatory network for nitrogen assimilation in Pseudomonas putida.恶臭假单胞菌中依赖NtrC的氮同化调控网络。
J Bacteriol. 2009 Oct;191(19):6123-35. doi: 10.1128/JB.00744-09. Epub 2009 Jul 31.
9
Mutations at pipX suppress lethality of PII-deficient mutants of Synechococcus elongatus PCC 7942.pipX 位点的突变可抑制聚球藻 PCC 7942 中 PII 缺陷型突变体的致死性。
J Bacteriol. 2009 Aug;191(15):4863-9. doi: 10.1128/JB.00557-09. Epub 2009 May 29.
10
Bacterial diversity of the broadbalk 'classical' winter wheat experiment in relation to long-term fertilizer inputs.与长期肥料投入相关的宽幅条播“经典”冬小麦试验的细菌多样性
Microb Ecol. 2008 Oct;56(3):525-37. doi: 10.1007/s00248-008-9372-0. Epub 2008 Mar 18.
本文引用的文献
1
The two opposing activities of adenylyl transferase reside in distinct homologous domains, with intramolecular signal transduction.腺苷酸转移酶的两种相反活性存在于不同的同源结构域中，并伴有分子内信号转导。
EMBO J. 1997 Sep 15;16(18):5562-71. doi: 10.1093/emboj/16.18.5562.
2
A family of ammonium transporters in Saccharomyces cerevisiae.酿酒酵母中的一个铵转运蛋白家族。
Mol Cell Biol. 1997 Aug;17(8):4282-93. doi: 10.1128/MCB.17.8.4282.
3
Evidence for two possible glnB-type genes in Herbaspirillum seropedicae.血清巴西固氮螺菌中两个可能的谷氨酰胺合成酶基因结合蛋白类型基因的证据。
J Bacteriol. 1997 Jul;179(14):4623-6. doi: 10.1128/jb.179.14.4623-4626.1997.
4
An alternative PII protein in the regulation of glutamine synthetase in Escherichia coli.大肠杆菌中谷氨酰胺合成酶调控中的一种替代PII蛋白。
Mol Microbiol. 1996 Jul;21(1):133-46. doi: 10.1046/j.1365-2958.1996.6281349.x.
5
Coexistence of two structurally similar but functionally different PII proteins in Azospirillum brasilense.巴西固氮螺菌中两种结构相似但功能不同的PII蛋白共存。
J Bacteriol. 1996 Jul;178(14):4143-9. doi: 10.1128/jb.178.14.4143-4149.1996.
6
Modulation of NifA activity by PII in Azospirillum brasilense: evidence for a regulatory role of the NifA N-terminal domain.巴西固氮螺菌中PII对NifA活性的调节：NifA N端结构域具有调控作用的证据
J Bacteriol. 1996 Aug;178(16):4830-8. doi: 10.1128/jb.178.16.4830-4838.1996.
7
Transcription of the glnB and glnA genes in the photosynthetic bacterium Rhodospirillum rubrum.光合细菌深红红螺菌中glnB和glnA基因的转录
Microbiology (Reading). 1996 May;142 ( Pt 5):1265-1272. doi: 10.1099/13500872-142-5-1265.
8
Azotobacter vinelandii NIFL is a flavoprotein that modulates transcriptional activation of nitrogen-fixation genes via a redox-sensitive switch.维涅兰德固氮菌NIFL是一种黄素蛋白，它通过氧化还原敏感开关调节固氮基因的转录激活。
Proc Natl Acad Sci U S A. 1996 Mar 5;93(5):2143-8. doi: 10.1073/pnas.93.5.2143.
9
Functional and genetic characterization of the (methyl)ammonium uptake carrier of Corynebacterium glutamicum.谷氨酸棒杆菌（甲基）铵摄取载体的功能和遗传特性
J Biol Chem. 1996 Mar 8;271(10):5398-403. doi: 10.1074/jbc.271.10.5398.
10
Nitrogen control in bacteria.细菌中的氮控制
Microbiol Rev. 1995 Dec;59(4):604-22. doi: 10.1128/mr.59.4.604-622.1995.
Zotero 插件