Van der Laken C J, Boerman O C, Oyen W J, Van de Ven M T, Ven der Meer J W, Corstens F H
Department of Nuclear Medicine, University Hospital Nijmegen, The Netherlands.
Nucl Med Commun. 1998 Mar;19(3):271-81. doi: 10.1097/00006231-199803000-00012.
Radiolabelled interleukin-8 (IL-8) is a promising agent for the imaging of infection and inflammation. Several experiments were performed to explore further the imaging potential of radiolabelled IL-8. IL-8 was radioiodinated via the Bolton-Hunter method. Rabbits with focal infection (Escherichia coli, Staphylococcus aureus) or sterile inflammation (zymosan) were injected intravenously with 18.5 MBq (0.5 mCi) of 123I-IL-8. In separate studies, rabbits were injected intravenously with 111In-granulocytes with or without 125I-IL-8. Gamma camera images were obtained at 5 min, 1, 4 and 8 h post-injection (p.i.). Biodistribution was determined at 8 h p.i. In all models, the biodistribution of 123I-IL-8 was characterized by rapid blood clearance and high uptake in infection and sterile inflammation. All foci could be clearly visualized within 4 h p.i. Ex vivo abscess-to-contralateral muscle ratios increased to 114.7+/-23.0 (E. coli), 52.3+/-24.5 (S. aureus) and 49.8+/-8.3 (zymosan) at 8 h p.i. In the circulation, most 123I-IL-8 was bound to erythrocytes. The abscess uptake of 125I-IL-8 reached high levels despite reduced migration of granulocytes towards the site of infection due to the anti-inflammatory activity of intravenously injected IL-8. IL-8 could be injected without induction of neutropenia at a dosage of 2 ng kg(-1). In conclusion, the characteristics of radiolabelled IL-8 for imaging of infection and sterile inflammation are highly encouraging and warrant further optimization for clinical application.
放射性标记的白细胞介素-8（IL-8）是一种用于感染和炎症成像的有前景的试剂。进行了多项实验以进一步探索放射性标记的IL-8的成像潜力。通过博尔顿-亨特法对IL-8进行放射性碘化。将患有局灶性感染（大肠杆菌、金黄色葡萄球菌）或无菌性炎症（酵母聚糖）的兔子静脉注射18.5 MBq（0.5 mCi）的123I-IL-8。在单独的研究中，给兔子静脉注射111In-粒细胞，同时或不同时注射125I-IL-8。在注射后（p.i.）5分钟、1、4和8小时获得γ相机图像。在注射后8小时测定生物分布。在所有模型中，123I-IL-8的生物分布特征是血液清除迅速，在感染和无菌性炎症中摄取高。在注射后4小时内所有病灶均可清晰显影。在注射后8小时，离体脓肿与对侧肌肉的比值分别增至114.7±23.0（大肠杆菌）、52.3±24.5（金黄色葡萄球菌）和49.8±8.3（酵母聚糖）。在循环中，大多数123I-IL-8与红细胞结合。尽管由于静脉注射IL-8的抗炎活性导致粒细胞向感染部位的迁移减少，但125I-IL-8在脓肿中的摄取仍达到高水平。以2 ng kg(-1)的剂量注射IL-8不会引起中性粒细胞减少。总之，放射性标记的IL-8用于感染和无菌性炎症成像的特性非常令人鼓舞，值得进一步优化以用于临床应用。
