Oftung F, Borka E, Mustafa A S
Department of Vaccinology, National Institute of Public Health, Torhov, Oslo, Norway.
FEMS Immunol Med Microbiol. 1998 Apr;20(4):319-25. doi: 10.1111/j.1574-695X.1998.tb01142.x.
Mycobacterium tuberculosis reactive T cell clones were established from naturally converted PPD-positive healthy subjects and screened for proliferative reactivity against defined M. tuberculosis protein antigens of 16, 19, 65 (HSP65), and 71 (HSP70) kDa recombinantly expressed in Escherichia coli. Among the recombinant antigens tested, the M. tuberculosis 16-kDa protein antigen, as expressed from the lambda gt11 phage Y3155, was found to induce T cell proliferation. Crossreactivity studies showed that the epitope recognized was present in M. tuberculosis, M. africanum as well as the vaccine strain M. bovis BCG. The M. tuberculosis 16-kDa reactive T cell clone identified showed the CD4+, CD8- phenotype, secreted interferon-gamma upon antigen stimulation, and displayed major histocompatibility complex class II restricted cytotoxicity against M. tuberculosis pulsed macrophages. The results obtained suggest that the recombinant M. tuberculosis 16-kDa antigen can be recognized by human Th1 cells with potential relevance to protection.
结核分枝杆菌反应性T细胞克隆是从自然转化的PPD阳性健康受试者中建立的，并针对在大肠杆菌中重组表达的16、19、65（HSP65）和71（HSP70）kDa的特定结核分枝杆菌蛋白抗原进行增殖反应筛选。在测试的重组抗原中，发现从λgt11噬菌体Y3155表达的结核分枝杆菌16-kDa蛋白抗原可诱导T细胞增殖。交叉反应研究表明，所识别的表位存在于结核分枝杆菌、非洲分枝杆菌以及疫苗株牛分枝杆菌卡介苗中。鉴定出的结核分枝杆菌16-kDa反应性T细胞克隆表现为CD4 +、CD8 - 表型，在抗原刺激下分泌干扰素-γ，并对负载结核分枝杆菌的巨噬细胞表现出主要组织相容性复合体II类限制性细胞毒性。所得结果表明，重组结核分枝杆菌16-kDa抗原可被人Th1细胞识别，可能与保护作用相关。
