Duan X, Li X, Xue Q, Abo-el-Saad M, Xu D, Wu R
Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, NY 14853, USA.
Nat Biotechnol. 1996 Apr;14(4):494-8. doi: 10.1038/nbt0496-494.
We introduced the potato proteinase inhibitor II (PINII) gene (pin2) into several Japonica rice varieties, and regenerated a large number of transgenic rice plants. Wound-inducible expression of the pin2 gene driven by its own promoter, together with the first intron of the rice actin 1 gene (act1), resulted in high-level accumulation of the PINII protein in the transgenic plants. The introduced pin2 gene was stably inherited in the second, third, and fourth generations, as shown by molecular analyses. Based on data from the molecular analyses, several homozygous transgenic lines were obtained. Bioassay for insect resistance with the fifth-generation transgenic rice plants showed that transgenic rice plants had increased resistance to a major rice insect pest, pink stem borer (Sesamia inferens). Thus, introduction of an insecticidal proteinase inhibitor gene into cereal plants can be used as a general strategy for control of insect pests.
我们将马铃薯蛋白酶抑制剂II（PINII）基因（pin2）导入多个粳稻品种，并再生出大量转基因水稻植株。由其自身启动子以及水稻肌动蛋白1基因（act1）的第一个内含子驱动的pin2基因伤口诱导型表达，导致PINII蛋白在转基因植株中高水平积累。分子分析表明，导入的pin2基因在第二代、第三代和第四代中稳定遗传。基于分子分析数据，获得了几个纯合转基因株系。对第五代转基因水稻植株进行的抗虫生物测定表明，转基因水稻植株对主要水稻害虫——二化螟（Sesamia inferens）的抗性增强。因此，将杀虫蛋白酶抑制剂基因导入谷类植物可作为控制害虫的通用策略。
