葡萄糖脱氢酶和氢化酶的体外产氢

In vitro hydrogen production by glucose dehydrogenase and hydrogenase.

作者信息

Woodward J, Mattingly S M, Danson M, Hough D, Ward N, Adams M

机构信息

Chemical Technology Division, Oak Ridge National Laboratory, TN 37831-6194, USA.

出版信息

Nat Biotechnol. 1996 Jul;14(7):872-4. doi: 10.1038/nbt0796-872.

DOI:10.1038/nbt0796-872

PMID:9631013

Abstract

A new in vitro enzymatic pathway for the generation of molecular hydrogen from glucose has been demonstrated. The reaction is based on the oxidation of glucose by Thermoplasma acidophilum glucose dehydrogenase with the concomitant oxidation of NADPH by Pyrococcus furiosus hydrogenase. Stoichiometric yields of hydrogen were produced from glucose with the continuous recycling of cofactor. This simple system may provide a method for the biological production of hydrogen from renewable sources. In addition, the other product of this reaction, gluconic acid, is a high-value chemical commodity.

摘要

一种新的从葡萄糖生成分子氢的体外酶促途径已得到证实。该反应基于嗜热栖热菌葡萄糖脱氢酶对葡萄糖的氧化以及激烈火球菌氢化酶对NADPH的伴随氧化。通过辅因子的持续循环，从葡萄糖中产生了化学计量产率的氢气。这个简单的系统可能为从可再生资源中生物制氢提供一种方法。此外，该反应的另一种产物葡萄糖酸是一种高价值的化学商品。