Yoshimura T, Yokoyama H, Fujii S, Takayama F, Oikawa K, Kamada H
Institute for Life Support Technology, Yamagata Technopolis Foundation, Japan.
Nat Biotechnol. 1996 Aug;14(8):992-4. doi: 10.1038/nbt0896-992.
Nitric oxide (NO), a simple diatomic free radical, is known to play a critical physiological role in diverse organisms. An iron complex, with N-(dithiocarboxy)sarcosine (Fe-DTCS), has a high affinity for endogenous NO and can trap, stabilize, and accumulate it. The stable NO adduct thus formed is detectable at room temperature with electron paramagnetic resonance (EPR) spectrometry. We report in vivo EPR imaging of endogenous NO, trapped by an Fe-DTCS complex, in the abdomen of a live mouse. To our knowledge, this is the first report on EPR imaging of endogenous free radicals produced in vivo. This EPR imaging method will be useful for the noninvasive investigation of the spatial distribution of NO in pathologic organs or tissues.
一氧化氮（NO）是一种简单的双原子自由基，已知在多种生物体中发挥关键的生理作用。一种含有N-（二硫代羧基）肌氨酸的铁配合物（Fe-DTCS），对内源性NO具有高亲和力，能够捕获、稳定并积累它。由此形成的稳定NO加合物在室温下可用电子顺磁共振（EPR）光谱法检测到。我们报告了在活体小鼠腹部对由Fe-DTCS配合物捕获的内源性NO进行的体内EPR成像。据我们所知，这是关于体内产生的内源性自由基的EPR成像的首次报告。这种EPR成像方法将有助于对病理器官或组织中NO的空间分布进行无创研究。
