Liang Z, Kuang J, Lin Z
Sun Yetsan Hospital, Zhongshan Medical University, Guangzhou.
Zhonghua Fu Chan Ke Za Zhi. 1997 Aug;32(8):467-70.
To evaluate the efficacy of gene therapy with the herpes simplex virus thymidine kinase (HSV-TK) gene transference followed by ganciclovir (GCV) therapy in ovarian cancer.
HSV-TK gene was inserted into the hind III site of pLNSX vector and recombinant plasmid (pLNS/HSV-TK) was constracted. Then, the recombinant plasmid was transfered into PA317 packaged cell and the producer cell line of recombinant retrovirus vector pLNS/HSV-TK was established. The cytotoxicity efficacy of HSV-TK/GCV system to AO cell line was evaluated by microcucture tetrajolium test (MTT) method.
The recombinant retroviral vector pLNS/HSK-TK can transfer HSV-SK gene into the genome of AO cell line and make it sensitive to GCV. The growth inhibitory rate of AO cell line transfered by pLNS/HSV-TK was 98.0% in the medium containing 400 mumol GVC.
The ovarian cancer cell line transfered by HSV-TK gene regressed rapidly in response to the GCV therapy.
评估单纯疱疹病毒胸苷激酶（HSV-TK）基因转染后联合更昔洛韦（GCV）治疗卵巢癌的疗效。
将HSV-TK基因插入pLNSX载体的Hind III位点，构建重组质粒（pLNS/HSV-TK）。然后，将重组质粒转入PA317包装细胞，建立重组逆转录病毒载体pLNS/HSV-TK的生产细胞系。采用四甲基偶氮唑盐微量酶反应比色法（MTT法）评估HSV-TK/GCV系统对AO细胞系的细胞毒性作用。
重组逆转录病毒载体pLNS/HSK-TK能将HSV-SK基因转入AO细胞系基因组，使其对GCV敏感。在含400μmol GVC培养基中，pLNS/HSV-TK转染的AO细胞系生长抑制率为98.0%。
HSV-TK基因转染的卵巢癌细胞系在GCV治疗后迅速消退。
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