过氧化物酶体增殖物激活受体γ（PPARγ）在大鼠主动脉平滑肌细胞中的表达。

Expression of peroxisome proliferator-activated receptor gamma (PPARgamma) in rat aortic smooth muscle cells.

作者信息

Iijima K, Yoshizumi M, Ako J, Eto M, Kim S, Hashimoto M, Sugimoto N, Liang Y Q, Sudoh N, Toba K, Ouchi Y

机构信息

Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Tokyo, Bunkyo-ku, 113-8655, Japan.

出版信息

Biochem Biophys Res Commun. 1998 Jun 18;247(2):353-6. doi: 10.1006/bbrc.1998.8794.

DOI:10.1006/bbrc.1998.8794

PMID:9642130

Abstract

Peroxisome proliferator-activated receptor gamma (PPARgamma), a member of nuclear receptors, is expressed at a high level in adipose tissue and plays an important role in adipocyte differentiation. In the present study, we identified the expression of PPARgamma in rat aortic smooth muscle cells (RASMC) using reverse transcription-polymerase chain reaction and gel mobility shift assay. In addition, to investigate whether PPARgamma in RASMC is functional or not, we examined the effect of two specific ligands for PPARgamma, a thiazolidinedione anti-diabetic agent, troglitazone, and 15-deoxy-Delta12,14-prostaglandin J2, on the transcriptional activity of PPAR responsive element (PPRE). A significant increase in the activity of PPRE by addition of these ligands was found. These results suggest that in RASMC, target genes for PPARgamma may be activated by specific ligands for PPARgamma through PPRE in their promoters. In conclusion, PPARgamma is expressed and functional in vascular smooth muscle cells.

摘要

过氧化物酶体增殖物激活受体γ（PPARγ）是核受体家族的一员，在脂肪组织中高表达，在脂肪细胞分化中起重要作用。在本研究中，我们使用逆转录-聚合酶链反应和凝胶迁移率变动分析鉴定了大鼠主动脉平滑肌细胞（RASMC）中PPARγ的表达。此外，为了研究RASMC中的PPARγ是否具有功能，我们检测了两种PPARγ特异性配体，一种噻唑烷二酮类抗糖尿病药物曲格列酮和15-脱氧-Δ12,14-前列腺素J2，对PPAR反应元件（PPRE）转录活性的影响。发现添加这些配体后PPRE的活性显著增加。这些结果表明，在RASMC中，PPARγ的靶基因可能通过其启动子中的PPRE被PPARγ的特异性配体激活。总之，PPARγ在血管平滑肌细胞中表达且具有功能。