猪球虫猪等孢球虫（Isospora suis Biester，1934）在细胞培养中的完全发育

Complete development of the porcine coccidium Isospora suis Biester, 1934 in cell cultures.

作者信息

Lindsay D S, Quick D P, Steger A M, Toivio-Kinnucan M A, Blagburn B L

机构信息

Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, Blacksburg 24061-0342, USA.

出版信息

J Parasitol. 1998 Jun;84(3):635-7.

PMID:9645875

Abstract

Development from inoculated sporozoites to unsporulated oocysts of Isospora suis Biester, 1934 is described in a swine testicular (ST) cell line. Sporozoites penetrated ST cells within 1 hr postinoculation (PI). Development was initially by endodyogeny to produce binucleate type I meronts and type I merozoites. Division by endodyogeny continued during the 13-day observation period and type I merozoites were the developmental stages most abundant at observation periods >3 days PI. Mutinucleate type II meronts and type II merozoites were first observed 7 days PI. Gamonts and oocysts were present 12 days PI. Oocysts did not sporulate in vitro. The ultrastructural features of stages were similar to those that occur in the pig host.

摘要

1934年猪等孢球虫从接种的子孢子发育为未孢子化卵囊的过程在猪睾丸（ST）细胞系中得到了描述。接种后1小时内，子孢子侵入ST细胞。最初通过内二分裂进行发育，产生双核I型裂殖体和I型裂殖子。在13天的观察期内，内二分裂持续进行，在接种后超过3天的观察期，I型裂殖子是最丰富的发育阶段。多核II型裂殖体和II型裂殖子在接种后7天首次观察到。配子体和卵囊在接种后12天出现。卵囊在体外未孢子化。各阶段的超微结构特征与在猪宿主中出现的特征相似。