Höppener J W, De Wit M J, Simarro-Doorten A Y, Roijers J F, van Herrewaarden H M, Lips C J, Parente F, Quincey D, Gaudray P, Khodaei S, Weber G, Teh B, Farnebo F, Larsson C, Zhang C X, Calender A, Pannett A A, Forbes S A, Bassett J H, Thakker R V, Lemmens I, Van de Ven W J, Kas K
Department of Internal Medicine, Utrecht University Hospital, The Netherlands.
Genomics. 1998 Jun 1;50(2):251-9. doi: 10.1006/geno.1998.5307.
In the process of identification of the multiple endocrine neoplasia type 1 gene, which was recently published, we isolated a novel gene in the 11q13 region. This gene (named ZFPL1, for zinc-finger protein-like 1) is expressed strongly in the exocrine pancreas as a 1.4-kb polyadenylated RNA encoding a putative protein of 310 amino acids. A mouse EST contig predicts an equally sized murine protein with 91% amino acid sequence identity to the human protein. No significant homology with known proteins could be found through database screening. However, zinc-finger-like domains and leucine-zipper-like motifs in the predicted ZFPL1 protein were identified, suggesting the presence of DNA-binding and dimerization domains possibly involved in transcription regulation. This notion is supported by the presence of a putative bipartite nuclear localization signal. This paper presents the full-length cDNA sequence for this gene, its genomic structure and chromosomal orientation, and expression studies by Northern blot hybridization and RNA in situ hybridization.
