Blomme E A, Sugimoto Y, McCauley L K, Lin Y C, Capen C C, Rosol T J
Department of Veterinary Biosciences, Ohio State University, Columbus 43210, USA.
Prostate. 1998 Jul 1;36(2):110-20. doi: 10.1002/(sici)1097-0045(19980701)36:2<110::aid-pros6>3.0.co;2-m.
Parathyroid hormone-related protein (PTHrP), a principal factor in the pathogenesis of humoral hypercalcemia of malignancy, is also widely expressed in many normal tissues, including human prostatic epithelial cells. The role of PTHrP in the prostate is not known, but may include regulation of cell growth and differentiation or calcium secretion into prostatic fluid. The dog is a valuable animal model for human prostatic diseases. The objective was to investigate the expression of PTHrP and the PTH/PTHrP (type 1) receptor in primary cultures of canine stromal and epithelial prostatic cells.
Expression and secretion of PTHrP and the PTH/PTHrP receptor was measured in homogeneous primary cultures of canine prostatic stromal and epithelial cells using immunohistochemistry, Northern blots, radioimmunoassay, RT-PCR, and receptor stimulation assays.
Epithelial and stromal cells expressed and secreted abundant PTHrP, but PTH/PTHrP receptor expression was not detected in either cell type.
PTHrP expression by stromal and epithelial prostatic cells and the absence of the PTH/PTHrP (type I) receptor suggest that some functions previously proposed for PTHrP in the prostate are unlikely. The separation procedure presented is a valuable tool for studying the role and regulation of PTHrP in the prostate.
甲状旁腺激素相关蛋白（PTHrP）是恶性肿瘤体液性高钙血症发病机制中的一个主要因素，它也在许多正常组织中广泛表达，包括人前列腺上皮细胞。PTHrP在前列腺中的作用尚不清楚，但可能包括调节细胞生长和分化或向前列腺液中分泌钙。狗是研究人类前列腺疾病的一种有价值的动物模型。目的是研究PTHrP和PTH/PTHrP（1型）受体在犬前列腺基质细胞和上皮细胞原代培养物中的表达情况。
采用免疫组织化学、Northern印迹、放射免疫分析、逆转录-聚合酶链反应（RT-PCR）和受体刺激试验，检测犬前列腺基质细胞和上皮细胞原代培养物中PTHrP和PTH/PTHrP受体的表达及分泌情况。
上皮细胞和基质细胞表达并分泌大量PTHrP，但在这两种细胞类型中均未检测到PTH/PTHrP受体的表达。
前列腺基质细胞和上皮细胞表达PTHrP，但缺乏PTH/PTHrP（I型）受体，这表明先前提出的PTHrP在前列腺中的一些功能不太可能存在。所介绍的分离方法是研究PTHrP在前列腺中的作用和调节的一种有价值的工具。
