小鼠BLM基因的cDNA克隆，该基因是人类布卢姆综合征基因的同源物，在睾丸中mRNA水平上高表达。

cDNA cloning of mouse BLM gene, the homologue to human Bloom's syndrome gene, which is highly expressed in the testis at the mRNA level.

作者信息

Seki T, Wang W S, Okumura N, Seki M, Katada T, Enomoto T

机构信息

Department of Molecular Cell Biology, Faculty of Pharmaceutical Sciences, Tohoku University, Sendai 980-8578, Japan.

出版信息

Biochim Biophys Acta. 1998 Jul 9;1398(3):377-81. doi: 10.1016/s0167-4781(98)00066-9.

DOI:10.1016/s0167-4781(98)00066-9

PMID:9655940

Abstract

We cloned a cDNA encoding the mouse homologue to human Bloom's syndrome gene (BLM). The deduced amino acid sequence of mouse Blm showed 76% identity to the human sequence with very high homology in seven consecutive domains characteristic of DNA and RNA helicases. The expression of mBLM mRNA was examined in various tissues. Extremely high expression was observed in the testis as compared with other tissues. The mBLM mRNA level in the testis began to increase 12-14 days after birth, corresponding to the appearance of cells in the pachytene phase.

摘要

我们克隆了一个编码小鼠同源人类布卢姆综合征基因（BLM）的cDNA。推导的小鼠Blm氨基酸序列与人类序列有76%的同一性，在DNA和RNA解旋酶特有的七个连续结构域中具有非常高的同源性。在各种组织中检测了mBLM mRNA的表达。与其他组织相比，在睾丸中观察到极高的表达。睾丸中的mBLM mRNA水平在出生后12 - 14天开始升高，这与粗线期细胞的出现相对应。