Wongsawad C, Rojanapaibul A, Vanittanakom P
Department of Biology, Faculty of Science, Faculty of Medicine, Chiang Mai University, Thailand.
Southeast Asian J Trop Med Public Health. 1997;28 Suppl 1:213-6.
Light and scanning electron microscopy of heterophyid fluke, Stellantchasmus sp were studied. The adults were obtained from Rattus norvegicus, 7 days after experimental infections with the metacercariae from the body cavity of Dermogenus pusillus. Living specimens and stained flukes were examined by light microscope. The present worm differed from S. batillans having a single testis while testes of S. gallinte were tandam. It is closed to S. falcatus and S. aspinosus but can not be identified to species because of acetabular spines. The tegumental surface by SEM was investigated on encysted metacercariae and adults. It was nearly smooth with one end shallow wide groove of the metacercarial surface. The entire surface of adult worms were almost covered with scale-like spines, pectinate, 7-9 teeth. The size of scale was largest in the middle of body and decreased both anterior and posterior. No spines were around the excretory pore but has a wrinkled surface appearance. The dorsal surface appeared to have scale-like spines and pectination on the ventral surface. The papillae consisted of a single club-like cilium, solitary or grouped on the anterior part of the body especially anterior of oral sucker. Acetabulum located slightly right of the middle and invagination.
对异形吸虫(Stellantchasmus sp)进行了光学显微镜和扫描电子显微镜研究。成虫取自挪威大鼠,在经微小隐翅虫体腔中的尾蚴进行实验感染7天后获得。通过光学显微镜检查活体标本和染色后的吸虫。当前这种吸虫与巴氏异形吸虫不同,巴氏异形吸虫有单个睾丸,而鸡异形吸虫的睾丸呈串联状。它与镰状异形吸虫和无棘异形吸虫相近,但由于腹吸盘棘而无法鉴定到种。通过扫描电子显微镜对包囊尾蚴和成虫的体表进行了研究。尾蚴体表几乎光滑,一端有浅而宽的凹槽。成虫的整个体表几乎都覆盖着栉状的鳞片状棘,有7 - 9个齿。鳞片大小在虫体中部最大,向前后两端减小。排泄孔周围无棘,但表面有皱纹。背面似乎有鳞片状棘,腹面有栉齿状结构。乳头由单个棒状纤毛组成,单个或成群分布在虫体前部,尤其是口吸盘前方。腹吸盘位于虫体中部略偏右处且有内陷。