非小细胞肺癌中的肿瘤浸润淋巴细胞是活化的T淋巴细胞。

Tumour-infiltrating lymphocytes in non-small cell lung cancer are activated T lymphocytes.

作者信息

Kuo S H, Chang D B, Lee Y C, Lee Y T, Luh K T

机构信息

Department of Laboratory Medicine, National Taiwan University Hospital, Taipei, ROC.

出版信息

Respirology. 1998 Mar;3(1):55-9. doi: 10.1046/j.1440-1843.1998.d01-9.x.

DOI:10.1046/j.1440-1843.1998.d01-9.x

PMID:9657662

Abstract

This study was carried out in order to investigate the local immune reaction of tumour-infiltrating lymphocytes (TILs) in the primary tumours of non-small cell lung carcinomas. Thirty non-small cell lung cancer (NSCLC) patients were included. The tumour tissue was taken at thoracotomy and monocellular suspension of the tumour was obtained by mechanical disaggregation. Dual-coloured flow cytometric analysis of TILs and their corresponding peripheral blood lymphocytes (PBLs) was performed. Tumour-infiltrating lymphocytes contained significantly higher proportions of CD3(+) T lymphocytes and CD8(+) T lymphocytes than the corresponding PBLs (82.0%+/-13.9% vs 66.3%+/-10.6% for CD3, P<0.001; 39.0%+/-18.4% vs 26.4%+/-5.2% for CD8, P<0.001). Tumour-infiltrating lymphocytes contained significantly higher proportions of activated memory lymphocytes than PBLs did (9.8%+/-8.6% vs 1.3%+/-1.5% for CD25, P<0.001; 40.5%+/-30.2% vs 10.2%+/-14.8% for CD71, P<0.001; 75.5%+/-11.9% vs 28.6%+/-9.8% for HLA-DR, P<0.001). These findings were also found in both CD4(+) TILs and CD8(+) TILs. The TILs of NSCLC contained higher proportions of T lymphocytes and CD8(+) lymphocytes than their corresponding peripheral bloods. The proportions of activated memory lymphocytes were also significantly higher in the TILs, both in CD4(+) TILs and CD8(+) TILs, than the corresponding PBLs.

摘要

本研究旨在调查非小细胞肺癌原发肿瘤中肿瘤浸润淋巴细胞（TILs）的局部免疫反应。纳入了30例非小细胞肺癌（NSCLC）患者。在开胸手术时获取肿瘤组织，并通过机械解离获得肿瘤单细胞悬液。对TILs及其相应的外周血淋巴细胞（PBLs）进行双色流式细胞术分析。肿瘤浸润淋巴细胞中CD3(+) T淋巴细胞和CD8(+) T淋巴细胞的比例显著高于相应的PBLs（CD3分别为82.0%±13.9%和66.3%±10.6%，P<0.001；CD8分别为39.0%±18.4%和26.4%±5.2%，P<0.001）。肿瘤浸润淋巴细胞中活化记忆淋巴细胞的比例显著高于PBLs（CD25分别为9.8%±8.6%和1.3%±1.5%，P<0.001；CD71分别为40.5%±30.2%和10.2%±14.8%，P<0.001；HLA-DR分别为75.5%±11.9%和28.6%±9.8%，P<0.001）。这些发现也在CD4(+) TILs和CD8(+) TILs中均有发现。NSCLC的TILs中T淋巴细胞和CD8(+)淋巴细胞的比例高于其相应的外周血。在CD4(+) TILs和CD8(+) TILs中，活化记忆淋巴细胞的比例也显著高于相应的PBLs。