Shibuki H, Katai N, Kuroiwa S, Kurokawa T, Yodoi J, Yoshimura N
Department of Ophthalmology, Shinshu University School of Medicine, Matsumoto, Japan.
Invest Ophthalmol Vis Sci. 1998 Jul;39(8):1470-7.
To evaluate the protective effects of recombinant adult T-cell leukemia- derived factor (ADF)-human thioredoxin against ischemia-reperfusion injury in the rat retina.
Retinal ischemia was induced in rats by increasing the intraocular pressure to 110 mm Hg for 60 minutes. Various doses of recombinant human ADF (rhADF) or vehicle were administered intravenously before ischemia induction and immediately after reperfusion. The degree of retinal damage was assessed by electroretinogram (ERG) recording, by measuring the inner retinal thickness, and by counting the number of TdT-dUTP terminal nick-end labeling (TUNEL)-positive cells in the inner nuclear layer.
The amplitudes of the ERG b-wave and oscillatory potentials were increased significantly by treatment before ischemia and after reperfusion with 0.5 mg or 5 mg rhADF and by treatment after reperfusion with 1 mg rhADF, compared with those of vehicle-treated control rats (P < 0.01). On day 28 after reperfusion, the thickness of the inner retina of control rats and of rats treated before ischemia and after reperfusion with 0.5 mg rhADF were 46.1+/-6.4 microm and 78.5+/-8.9 microm, respectively (P < 0.01). The number of TUNEL-positive cells on days 1 and 2 after reperfusion was decreased significantly by treatments with 0.5 mg rhADF compared with the number of TUNEL-positive cells in control rats (P < 0.01).
Electrophysiologic and histologic studies showed that ischemia for 60 minutes produces severe damage in vehicle-treated control rat retina, particularly in the inner retinal layer. Intravenous injection of rhADF protects the rat retina from ischemia-reperfusion injury.
评估重组成人T细胞白血病衍生因子（ADF）-人硫氧还蛋白对大鼠视网膜缺血再灌注损伤的保护作用。
通过将眼压升高至110 mmHg持续60分钟诱导大鼠视网膜缺血。在缺血诱导前和再灌注后立即静脉注射不同剂量的重组人ADF（rhADF）或赋形剂。通过视网膜电图（ERG）记录、测量视网膜内层厚度以及计数内核层中TdT-dUTP末端脱氧核苷酸转移酶介导的缺口末端标记（TUNEL）阳性细胞数量来评估视网膜损伤程度。
与赋形剂处理的对照大鼠相比，缺血前和再灌注后用0.5 mg或5 mg rhADF处理以及再灌注后用1 mg rhADF处理，ERG b波和振荡电位的振幅显著增加（P < 0.01）。再灌注后第28天，对照大鼠和缺血前及再灌注后用0.5 mg rhADF处理的大鼠视网膜内层厚度分别为46.1±6.4微米和78.5±8.9微米（P < 0.01）。与对照大鼠中TUNEL阳性细胞数量相比，再灌注后第1天和第2天用0.5 mg rhADF处理使TUNEL阳性细胞数量显著减少（P < 0.01）。
电生理和组织学研究表明，60分钟的缺血对赋形剂处理的对照大鼠视网膜造成严重损伤，尤其是视网膜内层。静脉注射rhADF可保护大鼠视网膜免受缺血再灌注损伤。
