发育过程中大鼠视网膜水平细胞中的钙结合蛋白-D 28 kD和小白蛋白

Calbindin-D 28 kD and parvalbumin in the horizontal cells of rat retina during development.

作者信息

Oguni M, Setogawa T, Shinohara H, Kato K

机构信息

Department of Ophthalmology, Shimane Medical University, Izumo, Japan.

出版信息

Curr Eye Res. 1998 Jun;17(6):617-22.

PMID:9663851

Abstract

PURPOSE

Calbindin-D 28K (CaD) and parvalbumin (PV) are calcium-binding proteins thought to act as an intraneural calcium ion buffering system in the central nervous system. We previously reported that PV appears at birth in some cells in the outer portion of the nuclear layer of the retina that can differentiate into horizontal cells. CaD is also distributed in horizontal cells; however, it is not clear at which developmental stage CaD appears.

METHODS

The development of horizontal cells and the distribution of CaD and PV was examined ultrastructurally and immunohistochemically in rat retinas from birth (P0) to postnatal day 50 (P50).

RESULTS

Our ultrastructural data showed clear differentiation of horizontal cells from other retinal cells in the early postnatal days. These cells became nearly mature around P10 to P12. PV appeared in the outer nuclear layer at birth (P0) and, by P7, was observed in the ganglion cell layer, amacrine cells, and horizontal cells. CaD-immunoreactive cells, however, were not seen in any portions of the retina until P7. At P10, CaD-immunoreactivity was faintly observed in horizontal cells, and its immunoreactivity gradually increased in the horizontal cells from P10 to P15 when the eyes of the rats opened. CaD was not found in the ganglion cell layer or in amacrine cells at any period examined.

CONCLUSION

In the horizontal cells, PV appeared when they were ultrastructurally distinguished from other retinal cells, while CaD appeared when they already had some structure for endocytosis and synaptic transmission, suggesting that PV and CaD may be good morphological and functional markers for horizontal cells, respectively.

摘要

目的

钙结合蛋白-D 28K（CaD）和小白蛋白（PV）是钙结合蛋白，被认为在中枢神经系统中作为神经内钙离子缓冲系统发挥作用。我们之前报道过，PV在出生时出现在视网膜核层外层的一些细胞中，这些细胞可分化为水平细胞。CaD也分布在水平细胞中；然而，CaD在哪个发育阶段出现尚不清楚。

方法

采用超微结构和免疫组织化学方法，研究出生（P0）至出生后50天（P50）大鼠视网膜中水平细胞的发育以及CaD和PV的分布。

结果

我们的超微结构数据显示，出生后早期水平细胞与其他视网膜细胞有明显分化。这些细胞在出生后10至12天左右接近成熟。PV在出生时（P0）出现在外核层，到出生后7天（P7），在神经节细胞层、无长突细胞和水平细胞中均可观察到。然而，直到P7，在视网膜的任何部位都未见到CaD免疫反应阳性细胞。在P10时，在水平细胞中可微弱观察到CaD免疫反应性，从P10到P15大鼠睁眼时，其在水平细胞中的免疫反应性逐渐增强。在任何检查时期，神经节细胞层和无长突细胞中均未发现CaD。