Grier R L, Zhao L, Adams C E, Wise G E
Department of Veterinary Anatomy and Cell Biology, School of Veterinary Medicine, Louisiana State University, Baton Rouge 70803-8408, USA.
Eur J Oral Sci. 1998 Jun;106(3):808-15. doi: 10.1046/j.0909-8836.1998.eos106307.x.
Tooth eruption requires the presence of a dental follicle around the unerupted tooth. Before the onset of eruption there is an influx of mononuclear cells into the follicle which, in turn, form osteoclasts that erode the alveolar bone. Eruption can be accelerated by the injection of colony-stimulating factor-one (CSF-1), a molecule that is maximally transcribed and translated in the dental follicle cells at the time of peak influx of mononuclear cells into the follicle of the rat first mandibular molar. To determine if the rat dental follicle cells secrete the CSF-1 needed for these cellular events, conditioned medium was collected from cultures of these cells. Using as a bioassay, a cell line (m-NFS 60) that is responsive to CSF-1 for growth, it was shown that conditioned medium from the follicle cells stimulated growth of the m-NFS 60 cells by almost 33% over the controls. Western blots confirmed that CSF-1 was secreted into the medium. Treating the dental follicle cells with an antisense oligodeoxynucleotide probe against CSF-1 reduced the amount of CSF-1 produced. These results demonstrate that CSF-1 is secreted by the dental follicle cells and that the production of CSF-1 can be reduced with an antisense probe. This secretion by the dental follicle might recruit mononuclear cells into the follicle to initiate tooth eruption.
牙齿萌出需要未萌出牙齿周围存在牙囊。在萌出开始前，单核细胞流入牙囊，这些单核细胞进而形成破骨细胞，侵蚀牙槽骨。注射集落刺激因子-1（CSF-1）可加速萌出，在大鼠第一下颌磨牙牙囊单核细胞流入高峰时，CSF-1分子在牙囊细胞中大量转录和翻译。为确定大鼠牙囊细胞是否分泌这些细胞事件所需的CSF-1，从这些细胞的培养物中收集条件培养基。使用对CSF-1有生长反应的细胞系（m-NFS 60）作为生物测定法，结果表明牙囊细胞的条件培养基使m-NFS 60细胞的生长比对照组提高了近33%。蛋白质印迹法证实CSF-1分泌到培养基中。用针对CSF-1的反义寡脱氧核苷酸探针处理牙囊细胞可减少CSF-1的产生量。这些结果表明CSF-1由牙囊细胞分泌，且反义探针可减少CSF-1的产生。牙囊的这种分泌可能会将单核细胞招募到牙囊中，从而启动牙齿萌出。
