大鼠Pax4的分子克隆：大鼠胰岛素瘤细胞中四种异构体的鉴定。
Molecular cloning of rat Pax4: identification of four isoforms in rat insulinoma cells.
作者信息
Tokuyama Y, Yagui K, Sakurai K, Hashimoto N, Saito Y, Kanatsuka A
机构信息
Second Department of Internal Medicine, Chiba University, School of Medicine, Japan.
出版信息
Biochem Biophys Res Commun. 1998 Jul 9;248(1):153-6. doi: 10.1006/bbrc.1998.8925.
Molecular cloning of rat Pax4 cDNA from a rat insulinoma cell line, RINm5F, library by PCR-based cloning strategy revealed four isoforms of the protein. Analysis of tissue distribution using Northern blotting and RT-PCR showed specific expression of Pax4 mRNA in pancreatic islets and RIN cells. RT-PCR confirmed that the mRNAs of four isoforms are expressed in RIN cells. These Pax4 variants may regulate the transcriptional activity of Pax4 during the development of pancreatic islets.
通过基于聚合酶链反应（PCR）的克隆策略，从大鼠胰岛素瘤细胞系RINm5F文库中克隆大鼠Pax4互补脱氧核糖核酸（cDNA），发现了该蛋白的四种异构体。利用Northern印迹法和逆转录-聚合酶链反应（RT-PCR）分析组织分布，结果显示Pax4信使核糖核酸（mRNA）在胰岛和RIN细胞中特异性表达。RT-PCR证实四种异构体的mRNA在RIN细胞中均有表达。这些Pax4变体可能在胰岛发育过程中调节Pax4的转录活性。
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