Yokoo A, Hirose T, Matsukawa M, Hotta H, Kunishima Y, Takahashi S
Department of Urology, Sapporo Medical University School of Medicine, Japan.
J Urol. 1998 Aug;160(2):592-6.
To investigate the role of intercellular adhesion molecule-1 (ICAM-1) in the renal inflammatory process, we studied the time-course fluctuation of ICAM-1 expression on inflammatory lesions in mice with experimentally induced bacterial pyelonephritis and the effect of in vivo administration of an anti-ICAM-1 monoclonal antibody (mAb) on leukocytic migration.
Ascending pyelonephritis was induced by transurethral instillation of Pseudomonas aeruginosa, and the expression of ICAM-1 in the pyelonephritic lesions was studied by immunohistochemical methods.
The expression of ICAM-1 on the pyelonephritic lesions closely paralleled the degree of infiltration of neutrophils and macrophages until 3 days after infection. At 7 days after infection, though the degree of infiltration of these cells was quite high, expression of ICAM-1 was reduced. Treatment with the anti-ICAM-1 mAb in mice with bacterial pyelonephritis resulted in suppression of influx of neutrophils and macrophages in the infected sites until 3 days after infection. However, at 7 days after infection inhibition of the influx of these cells was not seen.
These results suggest that ICAM-1 expression is transient and plays a key role in the influx of neutrophils and macrophages associated with the early-phase response, and that in the late phase ICAM-1 independent adhesion molecules may be more predominant.
为了研究细胞间黏附分子-1（ICAM-1）在肾脏炎症过程中的作用，我们研究了实验性诱导的细菌性肾盂肾炎小鼠炎症病变中ICAM-1表达的时间进程波动以及体内给予抗ICAM-1单克隆抗体（mAb）对白细胞迁移的影响。
通过经尿道灌注铜绿假单胞菌诱导上行性肾盂肾炎，并用免疫组化方法研究肾盂肾炎病变中ICAM-1的表达。
感染后3天内，肾盂肾炎病变中ICAM-1的表达与中性粒细胞和巨噬细胞的浸润程度密切平行。感染后7天，尽管这些细胞的浸润程度相当高，但ICAM-1的表达却降低了。用抗ICAM-1 mAb治疗细菌性肾盂肾炎小鼠，在感染后3天内可抑制感染部位中性粒细胞和巨噬细胞的流入。然而，感染后7天未见到这些细胞流入的抑制作用。
这些结果表明，ICAM-1的表达是短暂的，在与早期反应相关的中性粒细胞和巨噬细胞流入中起关键作用，并且在后期，ICAM-1非依赖性黏附分子可能更为主要。
