Pape H C, Grotz M, Remmers D, Dwenger A, Vaske R, Wisner D, Tscherne H
Department of Trauma Surgery, Hannover Medical School, Germany.
Intensive Care Med. 1998 Jun;24(6):590-8. doi: 10.1007/s001340050620.
To perform a reproducible long-term (10 days) large animal model of multiple systems organ failure without necessity of a continuous stimulus.
Adult female merino sheep submitted to a 5-day stimulation period followed by a 5-day observation period. Day 1: Hemorrhagic shock was combined with a traumatic surgical insult (reamed intramedullary femoral nailing), followed by serial administrations every 12 h for 5 days of a combination of endotoxin and zymosan activated plasma. Organ function was followed for 5 further days.
Cardiac index increased significantly during the study (day 1: 491 +/- 8 mm Hg; day 10: 427 +/- 20, p < 0.05). Liver function was impaired and bilirubin levels increased significantly (day 1: 2.9 +/- 0.3 micromol/l; day 10: 7.2 +/- 0.9; p < 0.05). Creatinine clearance decreased initially (day 1: 54 +/- 7 ml/min), increased to a peak on day 2 (104 +/- 27), and then deteriorated again (day 10: 53 +/- 18).
This new large animal model of trauma-induced MOF is reproducible and may be suitable for the study of new therapeutic approaches to therapy.
建立一种可重复的长期（10天）多系统器官衰竭的大型动物模型，无需持续刺激。
成年雌性美利奴绵羊，先进行5天的刺激期，然后是5天的观察期。第1天：失血性休克合并创伤性手术损伤（扩髓股骨髓内钉固定），随后每12小时连续给药5天，给予内毒素和酵母聚糖激活血浆的组合。对器官功能再进行5天的跟踪观察。
在研究期间心脏指数显著增加（第1天：491±8 mmHg；第10天：427±20，p<0.05）。肝功能受损，胆红素水平显著升高（第1天：2.9±0.3 μmol/l；第10天：7.2±0.9；p<0.05）。肌酐清除率最初下降（第1天：54±7 ml/min），在第2天升至峰值（104±27），然后再次恶化（第10天：53±18）。
这种新的创伤诱导多器官功能衰竭大型动物模型具有可重复性，可能适用于研究新的治疗方法。
