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本文引用的文献

1
Mechanisms of active transport in the FOF1 ATP synthase.FOF1 型 ATP 合酶中的主动运输机制。
J Membr Biol. 1996 May;151(2):101-11. doi: 10.1007/s002329900061.
2
A mechanism of proton translocation by F1F0 ATP synthases suggested by double mutants of the a subunit.由a亚基双突变体提出的F1F0 ATP合酶质子转运机制。
J Biol Chem. 1994 Dec 2;269(48):30364-9.
3
Limited differential mRNA inactivation in the atp (unc) operon of Escherichia coli.大肠杆菌atp（unc）操纵子中有限的差异mRNA失活
J Bacteriol. 1993 Sep;175(18):5791-7. doi: 10.1128/jb.175.18.5791-5797.1993.
4
Effects of the uncI gene on expression of uncB, the gene coding for the a subunit of the F1F0 ATPase of Escherichia coli.
FEBS Lett. 1995 Sep 4;371(2):127-31. doi: 10.1016/0014-5793(95)00867-9.
5
Degradation of Escherichia coli uncB mRNA by multiple endonucleolytic cleavages.通过多次核酸内切酶切割降解大肠杆菌uncB mRNA
J Bacteriol. 1995 Jul;177(14):3917-22. doi: 10.1128/jb.177.14.3917-3922.1995.
6
Use of the 'Perceptron' algorithm to distinguish translational initiation sites in E. coli.使用“感知器”算法区分大肠杆菌中的翻译起始位点。
Nucleic Acids Res. 1982 May 11;10(9):2997-3011. doi: 10.1093/nar/10.9.2997.
7
Stoichiometry of subunits in the H+-ATPase complex of Escherichia coli.大肠杆菌H⁺-ATP酶复合体中亚基的化学计量学
J Biol Chem. 1982 Feb 25;257(4):2009-15.
8
Overproduction of subunit a of the F0 component of proton-translocating ATPase inhibits growth of Escherichia coli cells.质子转运ATP酶F0组分的亚基a过量产生会抑制大肠杆菌细胞的生长。
J Bacteriol. 1984 Apr;158(1):300-6. doi: 10.1128/jb.158.1.300-306.1984.
9
The nucleotide sequence of the atp genes coding for the F0 subunits a, b, c and the F1 subunit delta of the membrane bound ATP synthase of Escherichia coli.编码大肠杆菌膜结合ATP合酶F0亚基a、b、c以及F1亚基δ的atp基因的核苷酸序列。
Mol Gen Genet. 1981;184(1):33-9. doi: 10.1007/BF00271191.
10
Differential polypeptide synthesis of the proton-translocating ATPase of Escherichia coli.大肠杆菌质子转运ATP酶的差异多肽合成
J Bacteriol. 1982 Sep;151(3):1363-71. doi: 10.1128/jb.151.3.1363-1371.1982.

鉴定一个影响大肠杆菌质子转运ATP酶（unc）操纵子uncB基因表达的基因内核糖体结合位点。

Identification of an intragenic ribosome binding site that affects expression of the uncB gene of the Escherichia coli proton-translocating ATPase (unc) operon.

作者信息

Matten S R, Schneider T D, Ringquist S, Brusilow W S

机构信息

Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA.

出版信息

J Bacteriol. 1998 Aug;180(15):3940-5. doi: 10.1128/JB.180.15.3940-3945.1998.

DOI:10.1128/JB.180.15.3940-3945.1998
PMID:9683492
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC107379/
Abstract

The uncB gene codes for the a subunit of the Fo proton channel sector of the Escherichia coli F1 Fo ATPase. Control of expression of uncB appears to be exerted at some step after translational initiation. Sequence analysis by the perceptron matrices (G. D. Stormo, T. D. Schneider, L. Gold, and A. Ehrenfeucht, Nucleic Acids Res. 10:2997-3011, 1982) identified a potential ribosome binding site within the uncB reading frame preceding a five-codon reading frame which is shifted one base relative to the uncB reading frame. Elimination of this binding site by mutagenesis resulted in a four- to fivefold increase in expression of an uncB'-'lacZ fusion gene containing most of uncB. Primer extension inhibition (toeprint) analysis to measure ribosome binding demonstrated that ribosomes could form an initiation complex at this alternative start site. Two fusions of lacZ to the alternative reading frame demonstrated that this site is recognized by ribosomes in vivo. The results suggest that expression of uncB is reduced by translational frameshifting and/or a translational false start at this site within the uncB reading frame.

摘要

uncB基因编码大肠杆菌F1F0ATP酶F0质子通道部分的α亚基。uncB表达的调控似乎在翻译起始后的某个步骤发挥作用。通过感知器矩阵进行的序列分析（G.D.斯托莫、T.D.施奈德、L.戈尔德和A.埃伦费赫特，《核酸研究》10:2997 - 3011，1982）在uncB阅读框内一个五密码子阅读框之前确定了一个潜在的核糖体结合位点，该五密码子阅读框相对于uncB阅读框偏移了一个碱基。通过诱变消除该结合位点导致包含uncB大部分序列的uncB'-'lacZ融合基因的表达增加了四到五倍。用于测量核糖体结合的引物延伸抑制（脚印）分析表明，核糖体可以在这个替代起始位点形成起始复合物。将lacZ与该替代阅读框的两个融合表明，该位点在体内被核糖体识别。结果表明，uncB的表达因uncB阅读框内该位点的翻译移码和/或翻译错误起始而降低。