Sutmuller M, Baelde H J, Tysma O M, de Heer E, Bruijn J A
Department of Pathology, Leiden University Hospital, Leiden, 2300 RC, The Netherlands.
Clin Immunol Immunopathol. 1998 Jul;88(1):114-22. doi: 10.1006/clin.1998.4561.
Chronic graft-versus-host disease (GvHD) in mice is a model resembling glomerulonephritis in human systemic lupus erythematosus. In the present study congenic mouse strains were used to investigate the pathogenetic role of (1) donor T cell subset chimerism and (2) donor thymocytes in this model. In GvHD employing minor lymphocyte-stimulating-1 (Mls-1)-compatible donors and recipients, full-blown immune complex glomerulonephritis was associated with a low-donor CD8(+) T cell chimerism. Injection of lymphocytes from Mls-1-negative donors (Mls-1(b)) into Mls-1-positive recipients (Mls-1(a)) induces a type of GvHD characterized by rapid self-limitation accompanied by the immediate inhibition of donor T cell chimerism and the absence of glomerulonephritis. However, omission of thymocytes from the donor inoculate does result in glomerular depositions containing immunoglobulins. These results suggest that donor CD8(+) T cell chimerism is associated with attenuation of immune complex glomerulonephritis, whereas Mls-1-incompatible donor T cell precursors prevent the disease.
小鼠慢性移植物抗宿主病（GvHD）是一种类似于人类系统性红斑狼疮中肾小球肾炎的模型。在本研究中，使用同基因小鼠品系来研究（1）供体T细胞亚群嵌合现象和（2）供体胸腺细胞在该模型中的致病作用。在采用次要淋巴细胞刺激-1（Mls-1）相容供体和受体的GvHD中，典型的免疫复合物性肾小球肾炎与低水平的供体CD8(+) T细胞嵌合现象相关。将来自Mls-1阴性供体（Mls-1(b)）的淋巴细胞注射到Mls-1阳性受体（Mls-1(a)）中，会引发一种GvHD，其特征为快速的自我限制，同时伴有供体T细胞嵌合现象的立即抑制以及无肾小球肾炎。然而，供体接种物中省略胸腺细胞确实会导致含有免疫球蛋白的肾小球沉积物。这些结果表明，供体CD8(+) T细胞嵌合现象与免疫复合物性肾小球肾炎的减轻相关，而Mls-1不相容的供体T细胞前体可预防该疾病。
