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[利用聚合酶链反应证实古巴存在人类T细胞嗜淋巴细胞病毒I型]

[Confirmation of the presence in Cuba of human T-cell lymphotropic virus type I using the polymerase chain reaction].

作者信息

Rolo Gómez F, De Armas M B, Mato Luis J, Lubián Caballero A L, Díaz Torres H

机构信息

Centro de Investigaciones Científicas de la Defensa Civil, San José de Las Lajas, provincia de La Habana, Cuba.

出版信息

Rev Cubana Med Trop. 1997;49(3):204-8.

PMID:9685988
Abstract

The first cases diagnoses in Cuba as HTLV-I/II seropositives (human T lymphotropic virus) were studied by polymerase chain reaction aimed at differentiating the type of virus causing the infection. 3 kits of primer oligonucleotides were used and the amplification products were detected by hybridization with specific oligoprobes. 100% of the cases were HTLV-I positives. No HTLV-II positivity was found. It was confirmed the presence in Cuba of this retrovirus, even though the seroprevalence is low if it is taken into consideration that the Caribbean is an endemic zone for HTLV-1.

摘要

对古巴首批被诊断为HTLV-I/II血清阳性(人类嗜T淋巴细胞病毒)的病例进行了聚合酶链反应研究,旨在区分引起感染的病毒类型。使用了3套引物寡核苷酸试剂盒,并通过与特异性寡核苷酸探针杂交检测扩增产物。100%的病例为HTLV-I阳性。未发现HTLV-II阳性。证实了这种逆转录病毒在古巴的存在,尽管考虑到加勒比地区是HTLV-1的流行区,其血清流行率较低。

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