Yazaki Y, Matsunaga S, Onishi T, Nagamine T, Origuchi N, Yamamoto T, Ishidou Y, Imamura T, Sakou T
Department of Orthopaedic Surgery, Faculty of Medicine, Kagoshima University, Japan.
Anticancer Res. 1998 Jul-Aug;18(4A):2339-44.
The expression of bone morphogenetic proteins (BMPs) and BMP receptors (BMPRs) in the epiphyseal growth plate has not been clarified. In this study, we studied immunohistochemically the spatial and temporal localization of BMP-2/4, osteogenic protein-1 (OP-1, or BMP-7), and BMP receptors (BMPR-IA, BMPR-IB, and BMPR-II) in the epiphyseal plate of growing rats. The proximal parts of tibia in growing rats were observed. At 12 weeks after birth, BMP-2/4 and OP-1 were expressed markedly in proliferating and maturing chondrocytes. BMPR-IA, IB and II were clearly co-expressed in proliferating and maturing chondrocytes, and the expression was decreased in hypertrophic chondrocytes. At 24 weeks, the expression of BMP-2/4 and OP-1 was decreased, but BMPRs were still well-expressed in proliferating chondrocytes. The temporal and spatial expression of BMP and BMPR suggests that BMP and BMP receptors play roles in the multistep cascade of enchondral ossification in the epiphyseal growth plate.
骨骺生长板中骨形态发生蛋白（BMPs）和BMP受体（BMPRs）的表达尚未明确。在本研究中，我们采用免疫组织化学方法研究了生长中大鼠骨骺板中BMP-2/4、成骨蛋白-1（OP-1，即BMP-7）和BMP受体（BMPR-IA、BMPR-IB和BMPR-II）的时空定位。观察了生长中大鼠胫骨的近端部分。出生后12周时，BMP-2/4和OP-1在增殖和成熟软骨细胞中显著表达。BMPR-IA、IB和II在增殖和成熟软骨细胞中明显共表达，而在肥大软骨细胞中表达降低。24周时，BMP-2/4和OP-1的表达下降，但BMPRs在增殖软骨细胞中仍表达良好。BMP和BMPR的时空表达表明，BMP和BMP受体在骨骺生长板软骨内成骨的多步骤级联反应中发挥作用。
