Senoo M, Seki N, Ohira M, Sugano S, Watanabe M, Inuzuka S, Okamoto T, Tachibana M, Tanaka T, Shinkai Y, Kato H
Department of Molecular Oncology, Nippon Roche Research Center, Kanagawa, Japan.
Biochem Biophys Res Commun. 1998 Jul 30;248(3):603-7. doi: 10.1006/bbrc.1998.9013.
The p53 protein, which regulates the rate of cell division and death, is the most frequently mutated tumor suppressor to be identified so far in human cancers. Recently, a gene with significant homology to p53, termed p73, has been identified in a chromosomal region that is implicated in the molecular pathogenesis of neuroblastoma. We have cloned a second human p53-related gene, termed p73L, which shows strong amino-acid similarity to p73. The p73L gene is mapped to human chromosome 3q27-28 using in situ hybridization technique. p73L encodes a protein of 586 amino acids and its putative DNA binding domain (DBD) has high identities to those of p53 (60.6%) and to p73 (87.8%). Northern blot analysis, which demonstrated that the expression profiles of p73L and p73 mRNAs are distinct in some tissues, implies that p73 and p73L may have separate, distinct roles in different tissues.
p53蛋白可调节细胞分裂和死亡速率，是目前在人类癌症中发现的最常发生突变的肿瘤抑制因子。最近，在与神经母细胞瘤分子发病机制相关的一个染色体区域上，发现了一个与p53具有显著同源性的基因，称为p73。我们克隆了第二个与人类p53相关的基因，称为p73L，它与p73表现出很强的氨基酸相似性。利用原位杂交技术，将p73L基因定位于人类染色体3q27 - 28。p73L编码一种由586个氨基酸组成的蛋白质，其假定的DNA结合结构域（DBD）与p53的相应结构域具有高度一致性（60.6%），与p73的相应结构域也具有高度一致性（87.8%）。Northern印迹分析表明，p73L和p73 mRNA在某些组织中的表达谱不同，这意味着p73和p73L可能在不同组织中发挥各自独特的作用。
