Oudot F, Cordelet C, Sergiel J P, Grynberg A
Lipides Membranaires et Fonctions Cardiovasculaires, INRA, Faculté des Sciences Pharmaceutiques et Biologiques, Paris, France.
Int J Vitam Nutr Res. 1998;68(4):263-71.
We studied the influence of membrane polyunsaturated fatty acids (PUFA) on prostanoid metabolism in the vascular endothelium, in pathophysiological conditions. Two models of cultured endothelial cells were used, from bovine aorta (BAEC) and human umbilical vein (HUVEC). In physiological conditions, the main prostanoids were prostacyclin and PGE2 in the BAEC and prostacyclin and PGF2 alpha in the HUVEC. Reoxygenation (2.5 hours) but not hypoxia (2.5 hours) enhanced prostanoid synthesis in both models. Cell enrichment with arachidonic acid (AA, n-6 cells) increased both AA and C22:4 n-6 and decreased n-3 PUFAs in the phospholipids. Conversely enrichment with eicosapentaenoic and docosahexaenoic acids (EPA and DHA, n-3 cells) increased the n-3 PUFAs and decreased the n-6 PUFAs. The BAEC incorporated more PUFA in the phospholipids than the HUVEC. Moreover in the n-3 cells, EPA incorporation was higher than that of DHA. Increasing AA increased the production of both prostacyclin and PGF2 alpha by the BAEC and only that of PGF2 alpha by the HUVEC. Increasing n-3 PUFA decreased the release of PGE2 and TxA2 by the BAEC and only that of prostacyclin by the HUVEC. In the n-6 cells, hypoxia became a stimulus for prostanoid production and the stimulating effect of reoxygenation was reinforced in the HUVEC whereas it was abolished in the BAEC. N-3 PUFA blocked the reoxygenation-stimulated production. These results suggest a strong importance of dietary PUFA in the response of vascular endothelium to pathological conditions.
我们研究了在病理生理条件下，膜多不饱和脂肪酸（PUFA）对血管内皮细胞中前列腺素代谢的影响。使用了两种培养的内皮细胞模型，分别来自牛主动脉（BAEC）和人脐静脉（HUVEC）。在生理条件下，BAEC中主要的前列腺素是前列环素和PGE2，而HUVEC中是前列环素和PGF2α。复氧（2.5小时）而非缺氧（2.5小时）增强了两种模型中的前列腺素合成。用花生四烯酸（AA，n-6细胞）富集细胞会增加磷脂中的AA和C22:4 n-6，并降低n-3多不饱和脂肪酸。相反，用二十碳五烯酸和二十二碳六烯酸（EPA和DHA，n-3细胞）富集会增加n-3多不饱和脂肪酸并降低n-6多不饱和脂肪酸。BAEC在磷脂中掺入的PUFA比HUVEC更多。此外，在n-3细胞中，EPA的掺入高于DHA。增加AA会增加BAEC中前列环素和PGF2α的产生，而在HUVEC中仅增加PGF2α的产生。增加n-3多不饱和脂肪酸会降低BAEC中PGE2和TxA2的释放，而在HUVEC中仅降低前列环素的释放。在n-6细胞中，缺氧成为前列腺素产生的刺激因素，复氧的刺激作用在HUVEC中增强，而在BAEC中则被消除。N-3多不饱和脂肪酸阻断了复氧刺激的产生。这些结果表明饮食中的PUFA在血管内皮细胞对病理状况的反应中具有重要意义。
