Ibarreta D, Urcelay E, Parrilla R, Ayuso M S
Department of Pathophysiology and Human Molecular Genetics, Centro de Investigaciones Biológicas (CSIC), Madrid, Spain.
Ann Neurol. 1998 Aug;44(2):216-22. doi: 10.1002/ana.410440212.
Epstein-Barr-transformed lymphocytes from Alzheimer's disease patients showed the following distinct features in controlling the intracellular pH compared with cells from normal age-matched controls: (1) The alphaIgM-induced intracellular acidification was more pronounced in Alzheimer's disease than control cells and this effect appears to be associated with a loss of effectiveness of a Ca2+/calmodulin-dependent mechanism in controlling the activity of the Na+/H+ exchanger; and (2) the intracellular H+-buffering capacity and the rate of proton efflux in response to an acid load were both decreased in Alzheimer's disease cells. It is concluded that the amplitude of the intracellular pH changes under acid-loading conditions will always be greater in Alzheimer's disease than in control cells.
与年龄匹配的正常对照者的细胞相比，阿尔茨海默病患者的爱泼斯坦-巴尔病毒转化淋巴细胞在控制细胞内pH方面表现出以下明显特征：(1)αIgM诱导的细胞内酸化在阿尔茨海默病患者中比对照细胞更明显，这种效应似乎与Ca2+/钙调蛋白依赖性机制控制Na+/H+交换器活性的有效性丧失有关；(2)阿尔茨海默病细胞的细胞内H+缓冲能力和对酸负荷的质子外流速率均降低。得出的结论是，在酸负荷条件下，阿尔茨海默病患者细胞内pH变化的幅度总是大于对照细胞。
