Odagiri Y, Uchida H
Division of Human and Health Sciences, Yamanashi Prefectural College of Nursing, Japan.
Mutat Res. 1998 Jul 8;415(1-2):35-45. doi: 10.1016/s1383-5718(98)00051-5.
The possible contribution of some selected serum micronutrients (beta-carotene, vitamins B12 and C, folic acid and alpha-tocopherol) to spontaneous chromosomal damage was investigated in human peripheral blood lymphocytes from 33 non-smoking healthy donors by the cytokinesis-block micronucleus assay. Labelling of micronuclei with antikinetochore serum was used to discriminate between kinetochore-positive and -negative micronuclei and thus between micronuclei which arise from whole chromosome loss and those which arise from chromosome breaks. Simple correlation analysis showed that age was significantly associated with the increased frequency of micronucleated cells, and this age-related increase in these cells was due to the increase in cells with both kinetochore-positive and -negative micronuclei. Serum micronutrient levels had no apparent significant effects on incidence of micronucleated cells except for the weak positive correlation between vitamin B12 levels and frequency of kinetochore-positive micronucleated cells. Multiple regression analysis with age and serum micronutrient levels as independent variables showed that (a) age was the most influential variable for the frequency of micronucleated cells, (b) the serum vitamin C level was associated with increased frequency of spontaneous micronucleated cells, and this increase was mainly due to the increase in cells with kinetochore-positive micronuclei, and (c) the serum folic acid level was significantly and negatively related to the frequencies of cells with both kinetochore-positive and -negative micronuclei. To avoid the predominant age-effect, we also performed separate multiple regression analysis with age-adjusted frequency of micronucleated cells as dependent variable. The results from this analysis again showed a significant and positive effect of serum vitamin C level on age-adjusted frequency of kinetochore-positive micronucleated cells, while marginal negative effect of folic acid on age-adjusted frequency of total micronucleated cells (P < 0.06) and kinetochore-positive micronucleated cells (P < 0.051) was detected. These results suggest that age and serum vitamin C are definitely variables for frequencies of spontaneous chromosome loss, and that serum folic acid is perhaps another important micronutrient which influence the frequency of spontaneous chromosomal damage.
通过胞质分裂阻滞微核试验，在33名不吸烟健康供者的人外周血淋巴细胞中，研究了某些选定的血清微量营养素（β-胡萝卜素、维生素B12和C、叶酸和α-生育酚）对自发染色体损伤的可能作用。使用抗动粒血清标记微核，以区分动粒阳性和阴性微核，从而区分由整条染色体丢失产生的微核和由染色体断裂产生的微核。简单相关分析表明，年龄与微核化细胞频率的增加显著相关，这些细胞中与年龄相关的增加是由于动粒阳性和阴性微核细胞的增加。血清微量营养素水平对微核化细胞的发生率没有明显的显著影响，除了维生素B12水平与动粒阳性微核细胞频率之间的弱正相关。以年龄和血清微量营养素水平作为自变量的多元回归分析表明：（a）年龄是微核化细胞频率最有影响的变量；（b）血清维生素C水平与自发微核化细胞频率的增加相关，这种增加主要是由于动粒阳性微核细胞的增加；（c）血清叶酸水平与动粒阳性和阴性微核细胞的频率显著负相关。为避免主要的年龄效应，我们还以年龄校正的微核化细胞频率作为因变量进行了单独的多元回归分析。该分析结果再次表明，血清维生素C水平对年龄校正的动粒阳性微核细胞频率有显著的正效应，而叶酸对年龄校正的总微核化细胞频率（P<0.06）和动粒阳性微核细胞频率（P<0.051）有边际负效应。这些结果表明，年龄和血清维生素C肯定是自发染色体丢失频率的变量，血清叶酸可能是另一种影响自发染色体损伤频率的重要微量营养素。
