Mandai M, Konishi I, Kuroda H, Nanbu K, Matsushita K, Yura Y, Hamid A A, Mori T
Department of Gynaecology and Obstetrics, Faculty of Medicine, Kyoto University, Japan.
Eur J Cancer. 1998 Apr;34(5):745-9. doi: 10.1016/s0959-8049(97)10147-2.
To elucidate the role of the FHIT (fragile histidine triad) gene in ovarian carcinogenesis, the expression of the gene was analysed by reverse transcription-polymerase chain reaction (RT-PCR) in 51 cases of ovarian carcinoma, 6 cases of borderline tumour and 4 cases of benign ovarian tumour. The concomitant expressions of normal and abnormal FHIT transcripts were detected in 39% of carcinomas and in 83% of borderline tumours, while benign tumours and normal ovarian tissues expressed only normal transcript. In addition, there were 4 (8%) cases of carcinoma lacking expression of normal FHIT transcript, all of which were in advanced stages (stage III-IV) and poorly differentiated. These results suggest that the expression of abnormal transcripts of the FHIT gene is a feature of ovarian malignant/borderline tumours and that the complete loss of normal FHIT expression is related to the progression of ovarian carcinoma in a subset of the cases. However, abnormal FHIT transcripts themselves were not associated with any clinicopathological parameters, such as clinical stage, histological subtype of tumour, grade of differentiation or outcome of the patient. Additionally, abnormal FHIT expression was not associated with the presence of loss of heterozygosity (LOH) at this locus, suggesting that abnormal FHIT transcripts are not derived from genetic alteration or that genetic alteration at this locus is complicated.
为阐明脆性组氨酸三联体（FHIT）基因在卵巢癌发生中的作用，采用逆转录-聚合酶链反应（RT-PCR）分析了51例卵巢癌、6例交界性肿瘤及4例卵巢良性肿瘤中该基因的表达情况。在39%的癌组织和83%的交界性肿瘤中检测到正常和异常FHIT转录本的同时表达，而良性肿瘤和正常卵巢组织仅表达正常转录本。此外，有4例（8%）癌组织缺乏正常FHIT转录本表达，均为晚期（Ⅲ-Ⅳ期）且分化差。这些结果表明，FHIT基因异常转录本的表达是卵巢恶性/交界性肿瘤的一个特征，在部分病例中，正常FHIT表达的完全缺失与卵巢癌的进展相关。然而，异常FHIT转录本本身与任何临床病理参数均无关联，如临床分期、肿瘤组织学亚型、分化程度或患者预后。此外，异常FHIT表达与该位点杂合性缺失（LOH）的存在无关，提示异常FHIT转录本并非源于基因改变，或该位点的基因改变较为复杂。
