Vaidyanathan S, Krishnan K R, Mansour P, Soni B M, McDicken I
Regional Spinal Injuries Centre, Southport, UK.
Spinal Cord. 1998 Aug;36(8):541-7. doi: 10.1038/sj.sc.3100589.
Nerve growth factor (NGF), apart from its role as a growth factor, appears to be involved in neuroimmune interactions and in tissue inflammation. Low-affinity nerve growth factor receptor (p75 NGFR), if demonstrated in the urothelium, could provide the means for (1) NGF-mediated modulation of the urothelial response to inflammation; (2) NGF-mediated autocrine/paracrine regulation of urothelial proliferation; and (3) p75 NGFR-mediated induction of apoptosis.
To investigate the presence of p75 NGFR in the vesical urothelium of patients with neuropathic bladder by immunohistochemical methods.
A hospital-based study of consecutive, unselected, adult patients of either sex with neuropathic bladder, undergoing procedure on the urinary tract in a Regional Spinal Injuries Centre located in the north-west of England.
Cold cup biopsies were taken from the trigone of the neuropathic urinary bladder of 26 patients with neuropathic bladder. Immunohistochemical studies were performed using antiNGF-receptor human monoclonal antibody which reacts with the low affinity receptor (p75 NGFR).
Both neural and epithelial structures showed positive immunostaining for p75 NGFR. The basal layer of the transitional epithelium showed strongly positive immunostaining for p75 NGFR in all the 26 cases. The luminal layer of transitional epithelium showed varying degree of positive immunostaining in 12 patients. The nerve fibres showed positive immunostaining for p75 NGFR. In many cases, the positively-stained nerve fibres were coursing very close to the basal layer of the urothelium almost entering the urothelium; however, no NGFR-positive intra-epithelial terminals could be seen. The positively-stained single nerve fibres and positively-stained thicker nerve bundles were seen in abundance in the submucosa but they were present in a sparse manner in the muscularis layer.
The presence of p75 NGFR was demonstrated in the urothelium of neuropathic bladder of all the 26 patients with neuropathic bladder. This observation may have potential therapeutic implications.
神经生长因子（NGF）除了作为一种生长因子发挥作用外，似乎还参与神经免疫相互作用和组织炎症。低亲和力神经生长因子受体（p75 NGFR）如果在尿路上皮中得到证实，可能为以下方面提供途径：（1）NGF介导的对尿路上皮炎症反应的调节；（2）NGF介导的尿路上皮增殖的自分泌/旁分泌调节；（3）p75 NGFR介导的细胞凋亡诱导。
通过免疫组织化学方法研究神经源性膀胱患者膀胱尿路上皮中p75 NGFR的存在情况。
一项基于医院的研究，对英格兰西北部一家地区脊髓损伤中心连续入选的、未经过挑选的成年神经源性膀胱患者（男女皆有）进行尿路手术。
对26例神经源性膀胱患者的神经源性膀胱三角区进行冷杯活检。使用与低亲和力受体（p75 NGFR）反应的抗NGF受体人单克隆抗体进行免疫组织化学研究。
神经和上皮结构对p75 NGFR均呈阳性免疫染色。在所有26例病例中，移行上皮的基底层对p75 NGFR呈强阳性免疫染色。12例患者的移行上皮腔面层呈不同程度的阳性免疫染色。神经纤维对p75 NGFR呈阳性免疫染色。在许多情况下，阳性染色的神经纤维非常靠近尿路上皮的基底层，几乎进入尿路上皮；然而，未见NGFR阳性的上皮内终末。阳性染色的单根神经纤维和较粗的阳性染色神经束在黏膜下层大量可见，但在肌层中分布稀疏。
在所有26例神经源性膀胱患者的神经源性膀胱尿路上皮中均证实存在p75 NGFR。这一观察结果可能具有潜在的治疗意义。
