Lerch P G, Spycher M O, Doran J E
ZLB Central Laboratory, Blood Transfusion Service SRC, Bern, Switzerland.
Thromb Haemost. 1998 Aug;80(2):316-20.
A reconstituted high density lipoprotein (rHDL) prepared for clinical use was tested for its influence on platelet activity modulated by various stimuli. In a first series of in vitro experiments, rHDL was added to blood in a concentration series, and platelet rich plasma (PRP) was isolated. Platelets were stimulated with arachidonic acid, collagen, epinephrine or ADP, and platelet aggregation was assessed. rHDL mediated a dose dependent inhibition of the platelet activity. With purified platelets rHDL inhibited the release reaction induced by collagen, but not by thrombin, as measured by CD62P (P-Selectin) expression on the plasma membrane. Ex vivo experiments were performed with PRP from volunteers, previously infused with 25 mg rHDL/kg body weight and 40 mg rHDL/kg body weight, respectively. Platelet activity in PRP was assessed before, and up to 30 h after the end of the rHDL infusion. A transient inhibition of the platelet aggregation induced by arachidonic acid and collagen was observed which was more pronounced in the group receiving 40 mg rHDL/kg body weight. In both groups of experiments, in vitro and ex vivo, the inhibition of the platelet activity was also dependent on the stimulus used.
对一种为临床使用而制备的重组高密度脂蛋白（rHDL）进行测试，以考察其对由各种刺激调节的血小板活性的影响。在第一系列体外实验中，将rHDL按浓度系列加入血液中，然后分离富含血小板血浆（PRP）。用花生四烯酸、胶原、肾上腺素或二磷酸腺苷刺激血小板，并评估血小板聚集情况。rHDL介导了对血小板活性的剂量依赖性抑制。对于纯化的血小板，rHDL抑制了由胶原诱导而非凝血酶诱导的释放反应，这通过质膜上CD62P（P-选择素）的表达来衡量。对分别预先输注25mg rHDL/kg体重和40mg rHDL/kg体重的志愿者的PRP进行了体内实验。在rHDL输注结束前及结束后长达30小时评估PRP中的血小板活性。观察到花生四烯酸和胶原诱导的血小板聚集出现短暂抑制，在接受40mg rHDL/kg体重的组中更为明显。在体外和体内两组实验中，对血小板活性的抑制也取决于所使用的刺激物。
