Growth associated protein 43 (GAP-43) mRNA is upregulated in the rat superior cervical ganglia after preganglionic transection.

Growth-associated protein 43 (GAP-43) is a growth-associated protein which is synthesised in high amounts in neurons during neuronal outgrowth. In a previous study we have shown that GAP-43 immunoreactivity is increased in neurons in superior cervical ganglia (SCG) and in nerve terminals in the irides after preganglionic denervation. We have now examined changes in GAP-43 mRNA using in situ hybridisation. GAP-43 mRNA was seen to be constitutively expressed by principal neurons of the rat superior cervical ganglion. Expression was increased further by section of the cervical sympathetic trunk, reaching a maximum (increased by about 30%) 3 days after decentralisation. The increased GAP-43 protein seen after decentralisation thus appears to be due to an upregulation of GAP-43 mRNA in the adrenergic neurons. The results imply that GAP-43 expression in the SCG is under presynaptic control, acting at least partly by control of mRNA levels.