Grindflek E, Sundvold H, Klungland H, Lien S
Agricultural University of Norway, Department of Animal Science, As, Norway.
Biochem Biophys Res Commun. 1998 Aug 28;249(3):713-8. doi: 10.1006/bbrc.1998.9212.
Two isoforms of peroxisome proliferator-activated receptor gamma (PPAR gamma) cDNAs, gamma 1 and gamma 2, have been isolated and characterised in swine. The relative expression of the two transcripts was studied by northern blot analysis using total RNA isolated from several porcine tissues taken at three different ages (day 1, after 5 weeks and at 100 kg weight). Hybridisation were carried out with two different probes, one binding to both PPAR gamma transcripts and the other being PPAR gamma 2 specific. Strongest hybridisation signals with the PPAR gamma probe binding both variants were detected in adipose tissues and spleen at all three ages, whereas only faint or no signals were detected in other tissues. The tissue distribution pattern of PPAR gamma 1 and gamma 2 suggests a modulation of tissue distribution for the two transcripts and obvious age and breed differences in gene expression in swine.
在猪中已分离并鉴定出两种过氧化物酶体增殖物激活受体γ（PPARγ）cDNA亚型，即γ1和γ2。使用从三个不同年龄（第1天、5周后和体重100千克时）采集的几种猪组织中分离的总RNA，通过Northern印迹分析研究了这两种转录本的相对表达。使用两种不同的探针进行杂交，一种与两种PPARγ转录本都结合，另一种是PPARγ2特异性的。在所有三个年龄的脂肪组织和脾脏中，检测到与两种变体都结合的PPARγ探针的最强杂交信号，而在其他组织中仅检测到微弱或无信号。PPARγ1和γ2的组织分布模式表明这两种转录本的组织分布存在调节，并且猪的基因表达存在明显的年龄和品种差异。
