Fidy J, Laberge M, Kaposi A D, Vanderkooi J M
Johnson Research Foundation, Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia 19104, USA.
Biochim Biophys Acta. 1998 Aug 18;1386(2):331-51. doi: 10.1016/s0167-4838(98)00101-0.
Fluorescence line narrowing is a high resolution spectroscopic technique that uses low temperature and laser excitation to optically select specific subpopulations from the inhomogeneously broadened absorption band of the sample. When applied to the study of fluorescent groups in proteins one can obtain vibronically resolved spectra, which can be analyzed to give information on spectral line shapes, vibrational energies of both the ground and excited state molecule, and the inhomogeneous distribution function of the electronic transitions. These parameters reveal information about the chromophoric prosthetic group and the protein matrix and are functions of geometric strains and local electric fields imposed by the protein. Examples of the use of fluorescence line narrowing are discussed in investigations of heme proteins, photosynthetic systems and tryptophan-containing proteins.
荧光谱线窄化是一种高分辨率光谱技术，它利用低温和激光激发从样品非均匀展宽的吸收带中光学选择特定的亚群。当应用于蛋白质中荧光基团的研究时，可以获得振转分辨光谱，通过分析该光谱可以得到谱线形状、基态和激发态分子的振动能量以及电子跃迁的非均匀分布函数等信息。这些参数揭示了发色辅基和蛋白质基质的信息，并且是由蛋白质施加的几何应变和局部电场的函数。在血红素蛋白、光合系统和含色氨酸蛋白的研究中讨论了使用荧光谱线窄化的实例。
