卡麦角林和催乳素对大鼠颗粒细胞体外产生孕酮和雌二醇的调节作用

In vitro production of progesterone and estradiol by rat granulosa cells regulated by cabergoline and prolactin.

作者信息

Negishi H, Furuta I, Ushigoe K, Fujimoto S, Koide S S

机构信息

Dept. of Obstetrics and Gynecology, Hokkaido University School of Medicine, Sapporo, Japan.

出版信息

Endocr Res. 1998 May;24(2):195-203. doi: 10.1080/07435809809135528.

DOI:10.1080/07435809809135528

PMID:9738697

Abstract

Cabergoline (CG), a dopamine agonist, and prolactin (PRL) added in vitro to isolated granulosa cells from immature rats cultured in serum-free medium inhibited FSH-stimulated production of progesterone. The lowest effective concentrations of CG and PRL were 10(-8)M and 1 ng/mL, respectively. In combination, the inhibitory activities were additive. CG at 10(-8) M also suppressed estradiol production by isolated rat granulosa cells; whereas PRL did not. In conclusion, CG inhibits FSH-stimulated progesterone and estradiol production by rat granulosa cells.

摘要

卡麦角林（CG）是一种多巴胺激动剂，在体外将其与催乳素（PRL）添加到在无血清培养基中培养的未成熟大鼠分离的颗粒细胞中，可抑制促卵泡激素（FSH）刺激的孕酮生成。CG和PRL的最低有效浓度分别为10^(-8)M和1 ng/mL。联合使用时，抑制活性具有相加性。10^(-8) M的CG也可抑制分离的大鼠颗粒细胞的雌二醇生成；而PRL则不能。总之，CG可抑制FSH刺激的大鼠颗粒细胞孕酮和雌二醇生成。