Betton G R, Owen L N
Br J Cancer. 1976 Oct;34(4):374-80. doi: 10.1038/bjc.1976.180.
Canine melanoma has been transplanted to allogeneic neonatal recipients receiving continuous immunosuppression with anti-lymphocyte serum. One spontaneous melanoma was directly transplanted into 8 recipients, 6 of which developed tumours. 5/5 melanoma cell cultures were transplantable, with 19 tumour takes in 31 allogeneic recipients. Serial passage was performed in the case of two melanomas. Tumour development required continuous immunosuppression and the site was dependent upon the route of inoculation and other factors. Transplanted cell cultures were all amelanotic in vitro and in vivo, except in the case of one melanoma which reverted to a melanotic morphology after in vivo growth.
犬黑色素瘤已被移植到接受抗淋巴细胞血清持续免疫抑制的同种异体新生受体中。一个自发性黑色素瘤被直接移植到8只受体动物体内，其中6只长出了肿瘤。5种黑色素瘤细胞培养物具有可移植性，在31只同种异体受体动物中有19只长出肿瘤。对两种黑色素瘤进行了连续传代。肿瘤的生长需要持续的免疫抑制，其生长部位取决于接种途径和其他因素。移植的细胞培养物在体外和体内均无黑色素生成，只有一种黑色素瘤在体内生长后恢复为黑色素生成形态。
