一种新的人类nm23同源物（nm23-H5）在睾丸生殖细胞中特异性表达。

A new human nm23 homologue (nm23-H5) specifically expressed in testis germinal cells.

作者信息

Munier A, Feral C, Milon L, Pinon V P, Gyapay G, Capeau J, Guellaen G, Lacombe M L

机构信息

INSERM U402, Faculté de Médecine Saint-Antoine, Paris, France.

出版信息

FEBS Lett. 1998 Sep 4;434(3):289-94. doi: 10.1016/s0014-5793(98)00996-x.

DOI:10.1016/s0014-5793(98)00996-x

PMID:9742940

Abstract

We have identified a cDNA encoding a 212 amino acid protein (Nm23-H5) with 27-31% identity to the human members of the nm23/nucleoside diphosphate (NDP) kinase gene family. The nm23-H5 gene is located on chromosome 5q23-31 and is transcribed as one main transcript of 1.1 kb which is highly and specifically expressed in testis, in the spermatogonia and early spermatocytes. Nm23-H5 possesses most of the residues conserved among NDP kinases plus an additional COOH-terminus end of 55 amino acids unique to this protein. However, under usual assay conditions, Nm23-H5 expressed in Escherichia coli did not exhibit NDP kinase activity. These results suggest that Nm23-H5 is specifically involved in early stages of spermatogenesis.

摘要

我们已鉴定出一种编码212个氨基酸蛋白质（Nm23-H5）的cDNA，它与nm23/核苷二磷酸（NDP）激酶基因家族的人类成员具有27%-31%的同源性。nm23-H5基因位于5号染色体的5q23-31区域，转录形成一个1.1kb的主要转录本，该转录本在睾丸、精原细胞和早期精母细胞中高度特异性表达。Nm23-H5拥有NDP激酶中大部分保守的残基，外加该蛋白质特有的一个55个氨基酸的额外羧基末端。然而，在常规检测条件下，在大肠杆菌中表达的Nm23-H5未表现出NDP激酶活性。这些结果表明，Nm23-H5特别参与精子发生的早期阶段。