Kantorow M, Horwitz J, Carper D
Jules Stein Eye Institute, Los Angeles, CA 90095-7065, USA.
Mol Vis. 1998 Sep 17;4:17.
To characterize gene expression patterns between epithelia isolated from cataractous and normal human lenses.
Reverse transcriptase differential display was used to identify differential expression between cataractous and normal epithelia. RT-PCR was used to compare pooled and individual RNA samples.
One transcript, up-regulated in cataractous as compared to normal epithelia, was identified as osteonectin which is also known as SPARC (secreted acidic protein rich in cysteines). RT-PCR confirmed over-expression of this RNA. High levels of osteonectin mRNA were also detected in six individual epithelia dissected from cataractous lenses.
The present study provides evidence for up-regulation of osteonectin in human age-related cataract and suggests that osteonectin, a protein involved in cell-cycle control, extracellular matrix and Ca++ binding, plays an important role in human lens homeostasis and may be involved in processes leading to lens opacity.
表征从白内障患者和正常人晶状体中分离出的上皮细胞之间的基因表达模式。
采用逆转录酶差异显示技术来鉴定白内障上皮细胞和正常上皮细胞之间的差异表达。使用RT-PCR比较混合的和单个的RNA样本。
与正常上皮细胞相比，在白内障上皮细胞中上调的一个转录本被鉴定为骨连接蛋白，其也被称为SPARC（富含半胱氨酸的分泌酸性蛋白）。RT-PCR证实了该RNA的过表达。在从白内障晶状体中分离出的六个单个上皮细胞中也检测到了高水平的骨连接蛋白mRNA。
本研究为骨连接蛋白在人类年龄相关性白内障中的上调提供了证据，并表明骨连接蛋白，一种参与细胞周期控制、细胞外基质和Ca++结合的蛋白质，在人类晶状体稳态中起重要作用，可能参与导致晶状体混浊的过程。
