伴放线放线杆菌产生的细胞周期特异性生长抑制因子是一种细胞致死性膨胀毒素。
The cell cycle-specific growth-inhibitory factor produced by Actinobacillus actinomycetemcomitans is a cytolethal distending toxin.
作者信息
Sugai M, Kawamoto T, Pérès S Y, Ueno Y, Komatsuzawa H, Fujiwara T, Kurihara H, Suginaka H, Oswald E
机构信息
Department of Microbiology, Hiroshima University School of Dentistry, Hiroshima 734-8553, Japan.
出版信息
Infect Immun. 1998 Oct;66(10):5008-19. doi: 10.1128/IAI.66.10.5008-5019.1998.
Actinobacillus actinomycetemcomitans has been shown to produce a soluble cytotoxic factor(s) distinct from leukotoxin. We have identified in A. actinomycetemcomitans Y4 a cluster of genes encoding a cytolethal distending toxin (CDT). This new member of the CDT family is similar to the CDT produced by Haemophilus ducreyi. The CDT from A. actinomycetemcomitans was produced in Escherichia coli and was able to induce cell distension, growth arrest in G2/M phase, nucleus swelling, and chromatin fragmentation in HeLa cells. The three proteins, CDTA, -B and -C, encoded by the cdt locus were all required for toxin activity. Antiserum raised against recombinant CDTC completely inhibited the cytotoxic activity of culture supernatant and cell homogenate fractions of A. actinomycetemcomitans Y4. These results strongly suggest that the CDT is responsible for the cytotoxic activity present in the culture supernatant and cell homogenate fractions of A. actinomycetemcomitans Y4. This CDT is a new putative virulence factor of A. actinomycetemcomitans and may play a role in the pathogenesis of periodontal diseases.
伴放线放线杆菌已被证明能产生一种不同于白细胞毒素的可溶性细胞毒性因子。我们在伴放线放线杆菌Y4中鉴定出了一组编码细胞致死性扩张毒素（CDT）的基因。CDT家族的这个新成员与杜克雷嗜血杆菌产生的CDT相似。伴放线放线杆菌的CDT在大肠杆菌中产生，能够诱导HeLa细胞出现细胞扩张、G2/M期生长停滞、细胞核肿胀和染色质碎片化。cdt基因座编码的三种蛋白质CDTA、-B和-C对毒素活性都是必需的。针对重组CDTC产生的抗血清完全抑制了伴放线放线杆菌Y4培养上清液和细胞匀浆组分的细胞毒性活性。这些结果有力地表明，CDT是伴放线放线杆菌Y4培养上清液和细胞匀浆组分中存在的细胞毒性活性的原因。这种CDT是伴放线放线杆菌一种新的假定毒力因子，可能在牙周疾病的发病机制中起作用。
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