Kayser-Wegmann I
Cell Tissue Res. 1976 Sep 1;171(4):513-21. doi: 10.1007/BF00220242.
Black cuticles of larvae and pupae from various Lepidoptera were studied by light and electron microscopy. There are striking differences in the representation of black pigmentation, especially at the ultrastructural level. Two types may be described: 1. With the light microscope black melanin-like grana, electron-dense electron microscopically, are found in the distal parts of the exocuticle. This type is demonstrated in larvae of Celerio euphorbiae, Papilio machaon, and Phalera bucephala. 2. With the light microscope, a dark homogeneous layer in the distal exocuticle can be recognized, however, electron microscopically no structures correlated with this dark pigment layer. This type of pigmentation was present in pupae of Pieris brassicae and Aglais urticae; in Pieris larvae the dark pigmented layer appeared to be limited to the epicuticle. In Celerio processes of the epidermal cells are involved in transporting precursors to the exocuticle. The conclusion was reached that black pigmentation in cuticles is based on different mechanisms as proposed by structural features. The two likely mechanisms are melanization and sclerotization.
通过光学显微镜和电子显微镜对各种鳞翅目幼虫和蛹的黑色表皮进行了研究。黑色色素沉着的表现存在显著差异，尤其是在超微结构水平上。可描述为两种类型：1. 用光学显微镜观察，在外皮的远端部分发现类似黑色黑色素的颗粒，电子显微镜下呈电子致密状。这种类型在大戟天蛾、金凤蝶和核桃美舟蛾的幼虫中得到证实。2. 用光学显微镜可以识别在外皮远端有一层深色均匀层，然而，电子显微镜下没有与该深色色素层相关的结构。这种色素沉着类型存在于菜粉蝶和荨麻蛱蝶的蛹中；在菜粉蝶幼虫中，深色色素层似乎仅限于表皮。在大戟天蛾中，表皮细胞的过程参与将前体物质运输到外皮。得出的结论是，表皮中的黑色色素沉着基于结构特征所提出的不同机制。两种可能的机制是黑化作用和硬化作用。
