Bjørnsdottir I, Tjørnelund J, Hansen S H
Department of Analytical and Pharmaceutical Chemistry, The Royal Danish School of Pharmacy, Copenhagen.
Electrophoresis. 1998 Sep;19(12):2179-86. doi: 10.1002/elps.1150191223.
The use of nonaqueous electrophoresis media for the application of capillary electrophoresis in the analysis of food, pharmaceuticals and biological fluids is reviewed. Some of the applications are discussed in detail and the benefits of using nonaqueous media in these cases are outlined. Three new applications within pharmaceutical analyses are presented. In these methods either a simple sample pretreatment by dilution with methanol (determination of chlorhexidine in a cream) or selective on-line capillary electrophoresis mass spectrometry (methods for identification of seizure drugs or opium alkaloids) are used. The choice of organic solvents and electrolytes for nonaqueous capillary electrophoresis are discussed. Furthermore, validation data obtained using capillary electrophoresis based on the nonaqueous principle are listed and discussed.
综述了非水电泳介质在毛细管电泳用于食品、药物和生物流体分析中的应用。详细讨论了一些应用，并概述了在这些情况下使用非水介质的好处。介绍了药物分析中的三个新应用。在这些方法中，要么通过用甲醇稀释进行简单的样品预处理（乳膏中洗必泰的测定），要么使用选择性在线毛细管电泳质谱法（癫痫药物或鸦片生物碱的鉴定方法）。讨论了非水毛细管电泳中有机溶剂和电解质的选择。此外，列出并讨论了基于非水原理使用毛细管电泳获得的验证数据。
