诺卡氏菌属内酰胺酶生产菌头孢霉素基因簇的九个基因由三个启动子转录为大型mRNA，其中两个启动子位于双向启动子区域。
The nine genes of the Nocardia lactamdurans cephamycin cluster are transcribed into large mRNAs from three promoters, two of them located in a bidirectional promoter region.
作者信息
Enguita F J, Coque J J, Liras P, Martin J F
机构信息
Area of Microbiology, Faculty of Biology, University of León, 24071 León, Spain.
出版信息
J Bacteriol. 1998 Oct;180(20):5489-94. doi: 10.1128/JB.180.20.5489-5494.1998.
Abstract
The nine biosynthesis genes of the Nocardia lactamdurans cephamycin cluster are expressed as three different mRNAs initiating at promoters latp, cefDp, and pcbABp, as shown by low-resolution S1 nuclease protection assays and Northern blotting analysis. Bidirectional expression occurred from divergent promoters (latp and cefDp) located in a 629-bp intergenic region that contains three heptameric direct repeats similar to those recognized by members of the SARP (Streptomyces antibiotic regulatory proteins) family. The lat gene is transcribed in a single monocistronic transcript initiating at latp. A second unusually long polycistronic mRNA (more than 16 kb) corresponding to six biosynthesis genes (pcbAB, pcbC, cmcI, cmcJ, cefF, and cmcH) started at pcbABp. A third polycistronic mRNA corresponding to the cefD and cefE genes started at cefDp.
摘要
通过低分辨率S1核酸酶保护试验和Northern印迹分析表明，诺卡氏菌内酰胺杜兰头孢霉素簇的九个生物合成基因以三种不同的mRNA形式表达，分别起始于启动子latp、cefDp和pcbABp。双向表达发生在位于629 bp基因间区域的发散启动子（latp和cefDp），该区域包含三个七聚体直接重复序列，类似于被SARP（链霉菌抗生素调节蛋白）家族成员识别的序列。lat基因在起始于latp的单个单顺反子转录本中被转录。第二个异常长的多顺反子mRNA（超过16 kb）对应六个生物合成基因（pcbAB、pcbC、cmcI、cmcJ、cefF和cmcH），起始于pcbABp。第三个对应cefD和cefE基因的多顺反子mRNA起始于cefDp。
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