诺卡氏菌属内酰胺酶生产菌头孢霉素基因簇的九个基因由三个启动子转录为大型mRNA，其中两个启动子位于双向启动子区域。
The nine genes of the Nocardia lactamdurans cephamycin cluster are transcribed into large mRNAs from three promoters, two of them located in a bidirectional promoter region.
作者信息
Enguita F J, Coque J J, Liras P, Martin J F
机构信息
Area of Microbiology, Faculty of Biology, University of León, 24071 León, Spain.
出版信息
J Bacteriol. 1998 Oct;180(20):5489-94. doi: 10.1128/JB.180.20.5489-5494.1998.
Abstract
The nine biosynthesis genes of the Nocardia lactamdurans cephamycin cluster are expressed as three different mRNAs initiating at promoters latp, cefDp, and pcbABp, as shown by low-resolution S1 nuclease protection assays and Northern blotting analysis. Bidirectional expression occurred from divergent promoters (latp and cefDp) located in a 629-bp intergenic region that contains three heptameric direct repeats similar to those recognized by members of the SARP (Streptomyces antibiotic regulatory proteins) family. The lat gene is transcribed in a single monocistronic transcript initiating at latp. A second unusually long polycistronic mRNA (more than 16 kb) corresponding to six biosynthesis genes (pcbAB, pcbC, cmcI, cmcJ, cefF, and cmcH) started at pcbABp. A third polycistronic mRNA corresponding to the cefD and cefE genes started at cefDp.
摘要
通过低分辨率S1核酸酶保护试验和Northern印迹分析表明，诺卡氏菌内酰胺杜兰头孢霉素簇的九个生物合成基因以三种不同的mRNA形式表达，分别起始于启动子latp、cefDp和pcbABp。双向表达发生在位于629 bp基因间区域的发散启动子（latp和cefDp），该区域包含三个七聚体直接重复序列，类似于被SARP（链霉菌抗生素调节蛋白）家族成员识别的序列。lat基因在起始于latp的单个单顺反子转录本中被转录。第二个异常长的多顺反子mRNA（超过16 kb）对应六个生物合成基因（pcbAB、pcbC、cmcI、cmcJ、cefF和cmcH），起始于pcbABp。第三个对应cefD和cefE基因的多顺反子mRNA起始于cefDp。
相似文献
1
The nine genes of the Nocardia lactamdurans cephamycin cluster are transcribed into large mRNAs from three promoters, two of them located in a bidirectional promoter region.诺卡氏菌属内酰胺酶生产菌头孢霉素基因簇的九个基因由三个启动子转录为大型mRNA，其中两个启动子位于双向启动子区域。
J Bacteriol. 1998 Oct;180(20):5489-94. doi: 10.1128/JB.180.20.5489-5494.1998.
2
Transcriptional mapping of the genes encoding the early enzymes of the cephamycin biosynthetic pathway of Streptomyces clavuligerus.产黄青霉头孢霉素生物合成途径早期酶编码基因的转录图谱分析。
Gene. 1994 May 3;142(1):41-8. doi: 10.1016/0378-1119(94)90352-2.
3
Characterization and expression in Streptomyces lividans of cefD and cefE genes from Nocardia lactamdurans: the organization of the cephamycin gene cluster differs from that in Streptomyces clavuligerus.来自耐乳糖诺卡氏菌的cefD和cefE基因在变铅青链霉菌中的特性及表达：头霉素基因簇的组织形式与棒状链霉菌不同。
Mol Gen Genet. 1993 Jan;236(2-3):453-8. doi: 10.1007/BF00277148.
4
The cephamycin biosynthetic genes pcbAB, encoding a large multidomain peptide synthetase, and pcbC of Nocardia lactamdurans are clustered together in an organization different from the same genes in Acremonium chrysogenum and Penicillium chrysogenum.诺卡氏菌中编码大型多结构域肽合成酶的头孢霉素生物合成基因pcbAB和pcbC聚集在一起，其排列方式与产黄顶头孢霉和产黄青霉中的相同基因不同。
Mol Microbiol. 1991 May;5(5):1125-33. doi: 10.1111/j.1365-2958.1991.tb01885.x.
5
Amy as a reporter gene for promoter activity in Nocardia lactamdurans: comparison of promoters of the cephamycin cluster.将艾米作为诺卡氏菌中启动子活性的报告基因：头孢霉素簇启动子的比较。
Appl Environ Microbiol. 1997 Aug;63(8):2977-82. doi: 10.1128/aem.63.8.2977-2982.1997.
6
Role of the cmcH-ccaR intergenic region and ccaR overexpression in cephamycin C biosynthesis in Streptomyces clavuligerus.cmcH-ccaR 基因间隔区和 ccaR 过表达在棒状链霉菌头孢菌素 C 生物合成中的作用。
Appl Microbiol Biotechnol. 2013 Jul;97(13):5869-80. doi: 10.1007/s00253-013-4721-4. Epub 2013 Jan 30.
7
Inducing effect of diamines on transcription of the cephamycin C genes from the lat and pcbAB promoters in Nocardia lactamdurans.二胺对产氨棒状链霉菌中lat和pcbAB启动子的头霉素C基因转录的诱导作用。
J Bacteriol. 1999 Apr;181(8):2379-84. doi: 10.1128/JB.181.8.2379-2384.1999.
8
A gene encoding lysine 6-aminotransferase, which forms the beta-lactam precursor alpha-aminoadipic acid, is located in the cluster of cephamycin biosynthetic genes in Nocardia lactamdurans.编码赖氨酸6-氨基转移酶的基因位于产氨棒状链霉菌的头孢霉素生物合成基因簇中，该酶可形成β-内酰胺前体α-氨基己二酸。
J Bacteriol. 1991 Oct;173(19):6258-64. doi: 10.1128/jb.173.19.6258-6264.1991.
9
Characterization of the cefF gene of Nocardia lactamdurans encoding a 3'-methylcephem hydroxylase different from the 7-cephem hydroxylase.
Appl Microbiol Biotechnol. 1996 Jan;44(5):605-9. doi: 10.1007/BF00172492.
10
Characterization of the cmcH genes of Nocardia lactamdurans and Streptomyces clavuligerus encoding a functional 3'-hydroxymethylcephem O-carbamoyltransferase for cephamycin biosynthesis.
Gene. 1995 Aug 30;162(1):21-7. doi: 10.1016/0378-1119(95)00308-s.
引用本文的文献
1
Roles of 2-oxoglutarate oxygenases and isopenicillin N synthase in β-lactam biosynthesis.2-氧代戊二酸氧酶和异青霉素 N 合酶在β-内酰胺生物合成中的作用。
Nat Prod Rep. 2018 Aug 15;35(8):735-756. doi: 10.1039/c8np00002f.
2
Transcriptional organization and regulation of magnetosome operons in Magnetospirillum gryphiswaldense.嗜铁钩端螺旋菌磁小体操纵子的转录组织与调控
Appl Environ Microbiol. 2006 Sep;72(9):5757-65. doi: 10.1128/AEM.00201-06.
3
Early cephamycin biosynthetic genes are expressed from a polycistronic transcript in Streptomyces clavuligerus.早期头孢霉素生物合成基因在棒状链霉菌中从一个多顺反子转录本表达。
J Bacteriol. 2000 Jan;182(2):348-56. doi: 10.1128/JB.182.2.348-356.2000.
4
Inducing effect of diamines on transcription of the cephamycin C genes from the lat and pcbAB promoters in Nocardia lactamdurans.二胺对产氨棒状链霉菌中lat和pcbAB启动子的头霉素C基因转录的诱导作用。
J Bacteriol. 1999 Apr;181(8):2379-84. doi: 10.1128/JB.181.8.2379-2384.1999.
本文引用的文献
1
A novel family of proteins that regulates antibiotic production in streptomycetes appears to contain an OmpR-like DNA-binding fold.一个调控链霉菌抗生素产生的新型蛋白质家族似乎含有一个类似OmpR的DNA结合结构域。
Mol Microbiol. 1997 Sep;25(6):1181-4. doi: 10.1046/j.1365-2958.1997.5421903.x.
2
A regulatory gene (ccaR) required for cephamycin and clavulanic acid production in Streptomyces clavuligerus: amplification results in overproduction of both beta-lactam compounds.产黄青霉中头孢霉素和克拉维酸生产所需的调控基因（ccaR）：扩增导致两种β-内酰胺化合物的过量生产。
J Bacteriol. 1997 Mar;179(6):2053-9. doi: 10.1128/jb.179.6.2053-2059.1997.
3
Overexpression of the Nocardia lactamdurans alpha-aminoadipyl-cysteinyl-valine synthetase in Streptomyces lividans. The purified multienzyme uses cystathionine and 6-oxopiperidine 2-carboxylate as substrates for synthesis of the tripeptide.诺卡氏菌内酰胺杜兰α-氨基己二酰-半胱氨酰-缬氨酸合成酶在淡紫链霉菌中的过表达。纯化的多酶以胱硫醚和6-氧代哌啶-2-羧酸为底物合成三肽。
Eur J Biochem. 1996 Dec 1;242(2):264-70. doi: 10.1111/j.1432-1033.1996.0264r.x.
4
Interaction of the two proteins of the methoxylation system involved in cephamycin C biosynthesis. Immunoaffinity, protein cross-linking, and fluorescence spectroscopy studies.
J Biol Chem. 1996 Dec 27;271(52):33225-30. doi: 10.1074/jbc.271.52.33225.
5
Characterization of the cefF gene of Nocardia lactamdurans encoding a 3'-methylcephem hydroxylase different from the 7-cephem hydroxylase.
Appl Microbiol Biotechnol. 1996 Jan;44(5):605-9. doi: 10.1007/BF00172492.
6
Genes for a beta-lactamase, a penicillin-binding protein and a transmembrane protein are clustered with the cephamycin biosynthetic genes in Nocardia lactamdurans.在耐内酰胺诺卡氏菌中，β-内酰胺酶、青霉素结合蛋白和跨膜蛋白的基因与头霉素生物合成基因聚集在一起。
EMBO J. 1993 Feb;12(2):631-9. doi: 10.1002/j.1460-2075.1993.tb05696.x.
7
Characterization and expression in Streptomyces lividans of cefD and cefE genes from Nocardia lactamdurans: the organization of the cephamycin gene cluster differs from that in Streptomyces clavuligerus.来自耐乳糖诺卡氏菌的cefD和cefE基因在变铅青链霉菌中的特性及表达：头霉素基因簇的组织形式与棒状链霉菌不同。
Mol Gen Genet. 1993 Jan;236(2-3):453-8. doi: 10.1007/BF00277148.
8
Transcriptional mapping of the genes encoding the early enzymes of the cephamycin biosynthetic pathway of Streptomyces clavuligerus.产黄青霉头孢霉素生物合成途径早期酶编码基因的转录图谱分析。
Gene. 1994 May 3;142(1):41-8. doi: 10.1016/0378-1119(94)90352-2.
9
A two-protein component 7 alpha-cephem-methoxylase encoded by two genes of the cephamycin C cluster converts cephalosporin C to 7-methoxycephalosporin C.由头霉素C基因簇的两个基因编码的一种双蛋白组分7α-头孢烯甲氧基化酶将头孢菌素C转化为7-甲氧基头孢菌素C。
J Bacteriol. 1995 Apr;177(8):2230-5. doi: 10.1128/jb.177.8.2230-2235.1995.
10
Characterization of the cmcH genes of Nocardia lactamdurans and Streptomyces clavuligerus encoding a functional 3'-hydroxymethylcephem O-carbamoyltransferase for cephamycin biosynthesis.
Gene. 1995 Aug 30;162(1):21-7. doi: 10.1016/0378-1119(95)00308-s.
Zotero 插件