Siddique A B, Ahlborg N, Wåhlin Flyg B, Perlmann P, Berzins K
Department of Immunology, Stockholm University, Sweden.
Parasitology. 1998 Sep;117 ( Pt 3):209-16. doi: 10.1017/s0031182098003023.
Antibodies to a non-repeat region of the Plasmodium falciparum antigen Pf155/RESA were investigated for their capacity to inhibit parasite cytoadherence to melanoma cells and parasite growth in vitro. The activities of these antibodies were studied since the target region in Pf155/RESA includes a cytoadherence-related motif also found in loop 3 and 7 of human erythrocyte band 3 protein. Overlapping multiple antigen peptides (MAPs) together spanning residues 199-220 of Pf155/RESA were used to raise antibodies in rabbits. Analysis of the fine specificity of these antibodies revealed that antibodies raised against largely overlapping sequences displayed highly different specificity patterns. Similarly, striking differences were seen when analysing the biological effect of antibodies to these MAPs. Antibodies to the cytoadherence-related motif of Pf155/RESA, as well as antibodies raised against a MAP based on a corresponding band 3 motif, inhibited cytoadherence but not parasite growth. In contrast, antibodies to sequences adjacent to the Pf155/RESA cytoadherence motif inhibited parasite growth in vitro but had no effect on cytoadherence.
对恶性疟原虫抗原Pf155/RESA非重复区域的抗体进行了研究，以考察其抑制寄生虫与黑色素瘤细胞细胞粘附及体外寄生虫生长的能力。对这些抗体的活性进行了研究，因为Pf155/RESA中的目标区域包含一个与人红细胞带3蛋白的环3和环7中也发现的细胞粘附相关基序。使用跨越Pf155/RESA第199 - 220位残基的重叠多抗原肽（MAPs）在兔中制备抗体。对这些抗体的精细特异性分析表明，针对高度重叠序列产生的抗体表现出高度不同的特异性模式。同样，在分析针对这些MAPs的抗体的生物学效应时也观察到了显著差异。针对Pf155/RESA细胞粘附相关基序的抗体，以及基于相应带3基序的MAPs产生的抗体，抑制细胞粘附但不抑制寄生虫生长。相反，针对Pf155/RESA细胞粘附基序相邻序列的抗体在体外抑制寄生虫生长，但对细胞粘附没有影响。
